Cell Cycle Antibodies 1

Anti-MAPRE1 Antibody (CAB2614)

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SKU:
CAB2614
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MAPRE1 Antibody
Antibody SKU:CAB2614
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 134-268 of human MAPRE1 (NP_036457.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:B-cell, U-87MG, LO2, HT-29, NIH/3T3, Mouse lung, Mouse spleen, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 134-268 of human MAPRE1 (NP_036457.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ETAV APSL VAPA LNKP KKPL TSSS AAPQ RPIS TQRT AAAP KAGP GVVR KNPG VGNG DDEA AELM QQVN VLKL TVED LEKE RDFY FGKL RNIE LICQ ENEG ENDP VLQR IVDI LYAT DEGF VIPD EGGP QEEQ EEY
Gene ID:22919
Uniprot:Q15691
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:29kDa
Observed MW:34kDa
Synonyms:MAPRE1, EB1
Background:The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.
UniProt Protein Function:EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.1-q11.23

Cellular Component: Golgi apparatus; centrosome; microtubule; cell projection membrane; cytoplasmic microtubule; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol

Molecular Function:protein C-terminus binding; microtubule plus-end binding; protein binding

Biological Process: mitosis; cell proliferation; cell division; organelle organization and biogenesis; negative regulation of microtubule polymerization; mitotic cell cycle; G2/M transition of mitotic cell cycle

NCBI Summary:The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
UniProt Code:Q15691
NCBI GenInfo Identifier:20138589
NCBI Gene ID:22919
NCBI Accession:Q15691.3
UniProt Related Accession:Q15691
Molecular Weight:
NCBI Full Name:Microtubule-associated protein RP/EB family member 1
NCBI Synonym Full Names:microtubule associated protein RP/EB family member 1
NCBI Official Symbol:MAPRE1  
NCBI Official Synonym Symbols:EB1  
NCBI Protein Information:microtubule-associated protein RP/EB family member 1
UniProt Protein Name:Microtubule-associated protein RP/EB family member 1
UniProt Synonym Protein Names:APC-binding protein EB1; End-binding protein 1; EB1
Protein Family:Microtubule-associated protein RP/EB family
UniProt Gene Name:MAPRE1  
UniProt Entry Name:MARE1_HUMAN
Anti-MAPRE1 Antibody (CAB2614)Western blot analysis of extracts of various cell lines, using MAPRE1 antibody (CAB2614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-MAPRE1 Antibody (CAB2614)Immunofluorescence analysis of HeLa cells using MAPRE1 antibody (CAB2614) at dilution of 1:100. Blue: DAPI for nuclear staining.
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