KO Validated Antibodies 2

Anti-MAPKAPK3 Antibody (CAB18095)[KO Validated]

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SKU:
CAB18095
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MAPKAPK3 Antibody [KO Validated]
Antibody SKU:CAB18095
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAPK3 (NP_004626.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAPK3 (NP_004626.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDGE TAEE QGGP VPPP VAPG GPGL GGAP GGRR EPKK YAVT DDYQ LSKQ VLGL GVNG KVLE CFHR RTGQ KCAL KLLY DSPK ARQE VDHH WQAS GGPH IVCI LDVY ENMH HGKR CLLI IMEC MEGG ELFS RIQE RGDQ AFTE REAA EIMR DIGT AIQF LHSH NIAH RDVK PENL LYTS KEKD AVLK LTDF GFAK ETTQ NALQ TPCY TPYY VAPE VLGP EKYD KSCD MWSL GVIM YILL CGFP
Gene ID:7867
Uniprot:Q16644
Cellular Location:Cytoplasm, Nucleus
Calculated MW:42kDa
Observed MW:38kDa
Anti-MAPKAPK3 Antibody [KO Validated] (CAB18095)Western blot analysis of extracts from normal (control) and MAPKAPK3 knockout (KO) HeLa cells, using MAPKAPK3 antibody (CAB18095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:MAPKAPK3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MDPT3, MK-3
Background:This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein.
UniProt Protein Function:MAPKAPK3: a member of the MAPKAPK family of protein kinases. Activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
UniProt Protein Details:

Protein type:Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; MAP kinase kinase activity; protein binding; protein serine/threonine kinase activity; signal transducer activity

Biological Process: activation of MAPK activity; cell surface receptor linked signal transduction; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; Ras protein signal transduction; response to cytokine stimulus; response to lipopolysaccharide; response to stress; signal transduction; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

NCBI Summary:This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
UniProt Code:Q16644
NCBI GenInfo Identifier:74762148
NCBI Gene ID:7867
NCBI Accession:Q16644.1
UniProt Secondary Accession:Q16644,B5BU67,
UniProt Related Accession:Q16644
Molecular Weight:
NCBI Full Name:MAP kinase-activated protein kinase 3
NCBI Synonym Full Names:mitogen-activated protein kinase-activated protein kinase 3
NCBI Official Symbol:MAPKAPK3  
NCBI Official Synonym Symbols:3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3  
NCBI Protein Information:MAP kinase-activated protein kinase 3
UniProt Protein Name:MAP kinase-activated protein kinase 3
UniProt Synonym Protein Names:Chromosome 3p kinase; 3pK
Protein Family:MAP kinase-activated protein kinase
UniProt Gene Name:MAPKAPK3  
UniProt Entry Name:MAPK3_HUMAN
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