Metabolism Antibodies 2

Anti-MAPKAP1 Antibody (CAB6648)

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SKU:
CAB6648
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-MAPKAP1 Antibody
Antibody SKU:CAB6648
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 370-486 of human MAPKAP1 (NP_077022.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, SGC-7901, A-549, Mouse testis, Mouse kidney, Mouse liver, Rat lung, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 370-486 of human MAPKAP1 (NP_077022.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GDKV EIDP VTNQ KAST KFWI KQKP ISID SDLL CACD LAEE KSPS HAIF KLTY LSNH DYKH LYFE SDAA TVNE IVLK VNYI LESR ASTA RADY FAQK QRKL NRRT SFSF QKEK KSGQ Q
Gene ID:79109
Uniprot:Q9BPZ7
Cellular Location:Cell membrane, Cytoplasmic vesicle, Nucleus, Peripheral membrane protein
Calculated MW:36kDa/37kDa/41kDa/53kDa/54kDa/59kDa
Observed MW:75kDa
Synonyms:MAPKAP1, JC310, MIP1, SIN1, SIN1b, SIN1g
Background:This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
UniProt Protein Function:Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function:protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; substantia nigra development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; T cell costimulation; innate immune response; negative regulation of Ras protein signal transduction; vascular endothelial growth factor receptor signaling pathway

NCBI Summary:This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BPZ7
NCBI GenInfo Identifier:15214282
NCBI Gene ID:79109
NCBI Accession:Q9BPZ7.2
UniProt Related Accession:Q9BPZ7
Molecular Weight:Observed: 59 kDaPredicted: 60 kDa
NCBI Full Name:Target of rapamycin complex 2 subunit MAPKAP1
NCBI Synonym Full Names:MAPK associated protein 1
NCBI Official Symbol:MAPKAP1  
NCBI Official Synonym Symbols:MIP1; SIN1; JC310; SIN1b; SIN1g  
NCBI Protein Information:target of rapamycin complex 2 subunit MAPKAP1
UniProt Protein Name:Target of rapamycin complex 2 subunit MAPKAP1
UniProt Synonym Protein Names:Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1
Protein Family:Stress-activated map kinase-interacting protein
UniProt Gene Name:MAPKAP1  
UniProt Entry Name:SIN1_HUMAN
Anti-MAPKAP1 Antibody (CAB6648)Western blot analysis of extracts of various cell lines, using MAPKAP1 antibody (CAB6648) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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