Cell Biology Antibodies 12

Anti-MAPK8IP1 Antibody (CAB9011)

(No reviews yet) Write a Review
SKU:
CAB9011
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MAPK8IP1 Antibody
Antibody SKU:CAB9011
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MAPK8IP1 (NP_005447.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, HT-1080, SKOV3, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MAPK8IP1 (NP_005447.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAER ESGG LGGG AASP PAAS PFLG LHIA SPPN FRLT HDIS LEEF EDED LSEI TDEC GISL QCKD TLSL RPPR AGLL SAGG GGAG SRLQ AEML QMDL IDAT GDTP GAED DEED DDEE RAAR RPGA GPPK AESG QEPA SRGQ GQSQ GQSQ GPGS GDTY RPKR PTTL NLFP QVPR SQDT LNNN SLGK KHSW QDRV SRSS SPLK TGEQ TPPH EHIC LSDE LPPQ SGPA PTTD RGTS TDSP CRRS TATQ MAPP GG
Gene ID:9479
Uniprot:Q9UQF2
Cellular Location:Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, perinuclear region
Calculated MW:77kDa
Observed MW:78kDa
Synonyms:MAPK8IP1, IB1, JIP-1, JIP1, PRKM8IP
Background:This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
UniProt Protein Function:JIP1: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade. Required for JNK activation in response to excitotoxic stress. Causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and by inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly or indirectly regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm

Molecular Function:kinesin binding; MAP-kinase scaffold activity; protein binding; protein kinase inhibitor activity

Biological Process: regulation of JNK cascade; vesicle-mediated transport

Disease: Diabetes Mellitus, Noninsulin-dependent

NCBI Summary:This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]
UniProt Code:Q9UQF2
NCBI GenInfo Identifier:17433093
NCBI Gene ID:9479
NCBI Accession:Q9UQF2.1
UniProt Secondary Accession:Q9UQF2,O43407, D3DQP4,
UniProt Related Accession:Q9UQF2
Molecular Weight:77,524 Da
NCBI Full Name:C-Jun-amino-terminal kinase-interacting protein 1
NCBI Synonym Full Names:mitogen-activated protein kinase 8 interacting protein 1
NCBI Official Symbol:MAPK8IP1  
NCBI Official Synonym Symbols:IB1; JIP1; JIP-1; PRKM8IP  
NCBI Protein Information:C-Jun-amino-terminal kinase-interacting protein 1
UniProt Protein Name:C-Jun-amino-terminal kinase-interacting protein 1
UniProt Synonym Protein Names:Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1
Protein Family:C-Jun-amino-terminal kinase-interacting protein
UniProt Gene Name:MAPK8IP1  
UniProt Entry Name:JIP1_HUMAN
Anti-MAPK8IP1 Polyclonal Antibody (CAB9011)Western blot analysis of extracts of various cell lines, using MAPK8IP1 antibody (CAB9011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
View AllClose

0 Reviews

View AllClose

Related Products