Name: Anti-MAP4K5 Antibody (CAB7962)
Brand: Cell Biology Antibodies 11
SKU: CAB7962
Availability: OutOfStock

Antibody Name:	MAP4K5 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7962
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-480 of human MAP4K5 (NP_006566.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, A-549, Jurkat, Mouse brain, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-480 of human MAP4K5 (NP_006566.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HAHY TEAD DDDF EPHA IIRH TIRS TNRN ARAE RTAS EINF DKLQ FEPP LRKE TEAR DEMG LSSD PNFM LQWN PFVD GANT GKST SKRA IPPP LPPK PRIS SYPE DNFP DEEK ASTI KHCP DSES RAPQ ILRR QSSP SCGP VAET SSIG NGDG ISKL MSEN TEGS AQAP QLPR KKDK RDFP K
Gene ID:	11183
Uniprot:	Q9Y4K4
Cellular Location:	Cytoplasm
Calculated MW:	95kDa
Observed MW:	120kDa

Synonyms:

MAP4K5, GCKR, KHS, KHS1, MAPKKKK5

Background:

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

UniProt Protein Function:	KHS1: a serine/threonine protein kinase of the STE20 family. Highly similar to yeast SPS1/STE20 kinase. Activates Jun kinase in mammalian cells, which suggested a role in stress response.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; KHS subfamily Chromosomal Location of Human Ortholog: 14q11.2-q21 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity Biological Process: protein amino acid phosphorylation; activation of JNK activity
NCBI Summary:	This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y4K4
NCBI GenInfo Identifier:	38570135
NCBI Gene ID:	11183
NCBI Accession:	NP_942089
UniProt Secondary Accession:	Q9Y4K4,Q8IYF6,
UniProt Related Accession:	Q9Y4K4
Molecular Weight:	95,040 Da
NCBI Full Name:	mitogen-activated protein kinase kinase kinase kinase 5
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase kinase kinase 5
NCBI Official Symbol:	MAP4K5
NCBI Official Synonym Symbols:	KHS; GCKR; KHS1; MAPKKKK5
NCBI Protein Information:	mitogen-activated protein kinase kinase kinase kinase 5
UniProt Protein Name:	Mitogen-activated protein kinase kinase kinase kinase 5
UniProt Synonym Protein Names:	Kinase homologous to SPS1/STE20; KHS; MAPK/ERK kinase kinase kinase 5; MEK kinase kinase 5; MEKKK 5
Protein Family:	Kievitone hydratase
UniProt Gene Name:	MAP4K5
UniProt Entry Name:	M4K5_HUMAN

Anti-MAP4K5 Polyclonal Antibody (CAB7962)

Western blot analysis of extracts of various cell lines, using MAP4K5 antibody (CAB7962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Antibodies

Anti-MAP4K5 Antibody (CAB15139)

MAP4K5 Antibody (PACO10375)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)