Name: Anti-MAP4K4 Antibody (CAB11706)
Brand: Cell Biology Antibodies 2
SKU: CAB11706
Availability: OutOfStock

Antibody Name:	Anti-MAP4K4 Antibody
Antibody SKU:	CAB11706
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_663719.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	U-87MG, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_663719.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EVLQ QQLL QEQA MLLH DHRR PHPQ HSQQ PPPP QQER SKPS FHAP EPKA HYEP ADRA REVE DRFR KTNH SSPE AQSK QTGR VLEP PVPS RSES FSNG NSES VHPA LQRP AEPQ VPVR TTSR SPVL SRRD SPLQ GSGQ QNSQ AGQR NSTS SIEP RLLW ERVE KLVP RPGS GSS
Gene ID:	9448
Uniprot:	O95819
Cellular Location:	Cytoplasm
Calculated MW:	133kDa/138kDa/141kDa/142kDa/145kDa/150kDa
Observed MW:	142kDa

Synonyms:

MAP4K4, FLH21957, HEL-S-31, HGK, MEKKK4, NIK

Background:

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily Chromosomal Location of Human Ortholog: 2q11.2-q12 Cellular Component: cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: microvillus biogenesis; regulation of catalytic activity; protein amino acid phosphorylation; regulation of JNK cascade
NCBI Summary:	The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	O95819
NCBI GenInfo Identifier:	29427585
NCBI Gene ID:	9448
NCBI Accession:	O95819.2
UniProt Secondary Accession:	O95819,O75172, Q9NST7,
UniProt Related Accession:	O95819
Molecular Weight:	1239
NCBI Full Name:	Mitogen-activated protein kinase kinase kinase kinase 4
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase kinase kinase 4
NCBI Official Symbol:	MAP4K4
NCBI Official Synonym Symbols:	HGK; NIK; MEKKK4; FLH21957; HEL-S-31
NCBI Protein Information:	mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; epididymis secretory protein Li 31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase
UniProt Protein Name:	Mitogen-activated protein kinase kinase kinase kinase 4
UniProt Synonym Protein Names:	HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase
Protein Family:	Mitogen-activated protein kinase kinase kinase kinase
UniProt Gene Name:	MAP4K4
UniProt Entry Name:	M4K4_HUMAN

Western blot analysis of extracts of various cell lines, using MAP4K4 antibody (CAB11706) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MAP4K4 Antibody (PACO00905)

MAP4K4 Antibody (PACO10374)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)