Name: Anti-MAP4K3 Antibody (CAB7351)
Brand: Cell Biology Antibodies 11
SKU: CAB7351
Availability: OutOfStock

Antibody Name:	Anti-MAP4K3 Antibody
Antibody SKU:	CAB7351
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 755-894 of human MAP4K3 (NP_003609.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, MCF7, 293T, HepG2, Mouse brain, Mouse kidney, Mouse lung, Rat heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 755-894 of human MAP4K3 (NP_003609.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ETVN PNST SSWF TESD TPQT NVTH VTQL ERDT ILVC LDCC IKIV NLQG RLKS SRKL SSEL TFDF QIES IVCL QDSV LAFW KHGM QGRS FRSN EVTQ EISD STRI FRLL GSDR VVVL ESRP TDNP TANS NLYI LAGH ENSY
Gene ID:	8491
Uniprot:	Q8IVH8
Cellular Location:
Calculated MW:	98kDa/100kDa/101kDa
Observed MW:	110kDa

Synonyms:	MAP4K3, GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1
Background:	This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; KHS subfamily Chromosomal Location of Human Ortholog: 2p22.1 Cellular Component: cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV
NCBI Summary:	This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q8IVH8
NCBI GenInfo Identifier:	29427817
NCBI Gene ID:	8491
NCBI Accession:	Q8IVH8.1
UniProt Secondary Accession:	Q8IVH8,Q6IQ39, Q8IVH7, Q9UDM5, Q9Y6R5,
UniProt Related Accession:	Q8IVH8
Molecular Weight:	Observed MW: 110kDaCalculated MW: 98kDa/100kDa/101kDa
NCBI Full Name:	Mitogen-activated protein kinase kinase kinase kinase 3
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol:	MAP4K3
NCBI Official Synonym Symbols:	GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
NCBI Protein Information:	mitogen-activated protein kinase kinase kinase kinase 3; MEK kinase kinase 3; MAPK/ERK kinase kinase kinase 3; germinal center kinase-like kinase; germinal center kinase-related protein kinase
UniProt Protein Name:	Mitogen-activated protein kinase kinase kinase kinase 3
UniProt Synonym Protein Names:	Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3
Protein Family:	Mitogen-activated protein kinase kinase kinase kinase
UniProt Gene Name:	MAP4K3
UniProt Entry Name:	M4K3_HUMAN

	Western blot analysis of extracts of various cell lines, using MAP4K3 antibody (CAB7351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using MAP4K3 antibody (CAB7351) at dilution of 1:100 (40x lens).