Cell Death Antibodies 1

Anti-MAP3K5 Antibody (CAB0283)

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SKU:
CAB0283
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-MAP3K5 Antibody
Antibody SKU:CAB0283
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1175-1374 of human MAP3K5 (NP_005914.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HL-60, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1175-1374 of human MAP3K5 (NP_005914.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RPHF SLAS ESDT ADQE DLDV EDDH EEQP SNQT VRRP QAVI EDAV ATSG VSTL SSTV SHDS QSAH RSLN VQLG RMKI ETNR LLEE LVRK EKEL QALL HRAI EEKD QEIK HLKL KSQP IEIP ELPV FHLN SSGT NTED SELT DWLR VNGA DEDT ISRF LAED YTLL DVLY YVTR DDLK CLRL RGGM LCTL WKAI IDFR NKQT
Gene ID:4217
Uniprot:Q99683
Cellular Location:Cytoplasm, Endoplasmic reticulum
Calculated MW:69kDa/154kDa
Observed MW:155kDa
Synonyms:MAP3K5, ASK1, MAPKKK5, MEKK5
Background:Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
UniProt Protein Function:ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.
UniProt Protein Details:

Protein type:EC 2.7.11.25; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: cytosol

Molecular Function:ATP binding; magnesium ion binding; MAP kinase kinase kinase activity; protein binding; protein homodimerization activity; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: activation of JNK activity; activation of MAPKK activity; induction of apoptosis by oxidative stress; JNK cascade; MAPKKK cascade; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of JNK activity; positive regulation of JNK cascade; protein amino acid phosphorylation

NCBI Summary:Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
UniProt Code:Q99683
NCBI GenInfo Identifier:6685617
NCBI Gene ID:4217
NCBI Accession:Q99683.1
UniProt Secondary Accession:Q99683,Q5THN3, Q99461, A6NIA0, B4DGB2,
UniProt Related Accession:Q99683
Molecular Weight:69,839 Da
NCBI Full Name:Mitogen-activated protein kinase kinase kinase 5
NCBI Synonym Full Names:mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol:MAP3K5  
NCBI Official Synonym Symbols:ASK1; MEKK5; MAPKKK5  
NCBI Protein Information:mitogen-activated protein kinase kinase kinase 5
UniProt Protein Name:Mitogen-activated protein kinase kinase kinase 5
UniProt Synonym Protein Names:Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
Protein Family:DASH complex
UniProt Gene Name:MAP3K5  
Anti-MAP3K5 Antibody (CAB0283)Western blot analysis of extracts of various cell lines, using MAP3K5 antibody (CAB0283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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