Cell Biology Antibodies 14

Anti-MAP3K4 Antibody (CAB16918)

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SKU:
CAB16918
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MAP3K4 Antibody
Antibody SKU:CAB16918
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Rat
Positive Samples:K-562, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MREA AAAL VPPP AFAV TPAA AMEE PPPP PPPP PPPP EPET ESEP ECCL AARQ EGTL GDSA CKSP ESDL EDFS DETN TENL YGTS PPST PRQM KRMS TKHQ
Gene ID:4216
Uniprot:Q9Y6R4
Cellular Location:
Calculated MW:177kDa/181kDa
Observed MW:170kDa
Anti-MAP3K4 Antibody (CAB16918)Western blot - MAP3K4 Rabbit pAb (CAB16918)
Synonyms:MAP3K4, MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412
Background:The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
UniProt Protein Function:MEKK4: a protein kinase of the STE11 family. A mediator of environmental stresses. Phosphorylates and activates MKK4, -6, and-7, which in turn activate the p38 AND JNK MAPK pathways.
UniProt Protein Details:

Protein type:Protein kinase, STE; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function:MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding

Biological Process: positive regulation of JNK activity; activation of MAPKK activity; regulation of gene expression; activation of MAPK activity; MAPKKK cascade; protein amino acid phosphorylation; response to UV-C; placenta development; male germ-line sex determination

NCBI Summary:The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]
UniProt Code:Q9Y6R4
NCBI GenInfo Identifier:296434576
NCBI Gene ID:4216
NCBI Accession:Q9Y6R4.2
UniProt Secondary Accession:Q9Y6R4,Q5VTT8, Q5VTT9, Q92612, Q9H408, A6H8W0, B7ZLD3 B9EG75,
UniProt Related Accession:Q9Y6R4
Molecular Weight:177,014 Da
NCBI Full Name:Mitogen-activated protein kinase kinase kinase 4
NCBI Synonym Full Names:mitogen-activated protein kinase kinase kinase 4
NCBI Official Symbol:MAP3K4  
NCBI Official Synonym Symbols:MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412  
NCBI Protein Information:mitogen-activated protein kinase kinase kinase 4; MEK kinase 4; MAP three kinase 1; MAP/ERK kinase kinase 4; MAPK/ERK kinase kinase 4; predicted protein of HQ0412; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of; dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)
UniProt Protein Name:Mitogen-activated protein kinase kinase kinase 4
UniProt Synonym Protein Names:MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4
Protein Family:Mitogen-activated protein kinase kinase kinase
UniProt Gene Name:MAP3K4  
UniProt Entry Name:M3K4_HUMAN
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