Antibody Name:	Anti-MAP3K3 Antibody
Antibody SKU:	CAB16058
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MAP3K3 (NP_976226.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, Mouse brain, Mouse liver, Rat pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MAP3K3 (NP_976226.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDEQ EALN SIMN DLVA LQMN RRHR MPGY ETMK NKDT GHSN RQKK HNSS SSAL LNSP TVTT SSCA GASE KKKF LSDV RIKF EHNG ERRI IAFS RPVK YEDV EHKV TTVF GQPL DLHY MNNE LSIL LKNQ DDLD KAID ILDR SSSM KSLR ILLL SQDR NHNS SSPH SGVS RQVR IKAS QSAG DINT IYQP PEPR SRHL SVSS QNPG RSSP PPGY VPER QQHI
Gene ID:	4215
Uniprot:	Q99759
Cellular Location:
Calculated MW:	70kDa/74kDa
Observed MW:	71kDa

Synonyms:

MAP3K3, MAPKKK3, MEKK3

Background:

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.
UniProt Protein Details:	Protein type:Protein kinase, STE; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family Chromosomal Location of Human Ortholog: 17q23.3 Cellular Component: intracellular; cytosol Molecular Function:MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade
NCBI Summary:	This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99759
NCBI GenInfo Identifier:	160332306
NCBI Gene ID:	4215
NCBI Accession:	Q99759.2
UniProt Secondary Accession:	Q99759,Q5BKZ6, Q8N3I9, B2RCW2, D3DU15,
UniProt Related Accession:	Q99759
Molecular Weight:	74,089 Da
NCBI Full Name:	Mitogen-activated protein kinase kinase kinase 3
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase kinase 3
NCBI Official Symbol:	MAP3K3
NCBI Official Synonym Symbols:	MEKK3; MAPKKK3
NCBI Protein Information:	mitogen-activated protein kinase kinase kinase 3; MEKK 3; MEK kinase 3; MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3
UniProt Protein Name:	Mitogen-activated protein kinase kinase kinase 3
UniProt Synonym Protein Names:	MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3
UniProt Gene Name:	MAP3K3
UniProt Entry Name:	M3K3_HUMAN

	Western blot analysis of extracts of various cell lines, using MAP3K3 antibody (CAB16058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MAP3K3 antibody (CAB16058) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using MAP3K3 antibody (CAB16058) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using MAP3K3 antibody (CAB16058) at dilution of 1:100 (40x lens).