Cell Biology Antibodies 12

Anti-MAP3K12 Antibody (CAB8464)

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SKU:
CAB8464
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MAP3K12 Antibody
Antibody SKU:CAB8464
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SH-SY5Y, HT-1080
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MACL HETR TPSP SFGG FVST LSEA SMRK LDPD TSDC TPEK DLTP TQCV LRDV VPLG GQGG GGPS PSPG GEPP PEPF ANSV LQLH EQDA GGPG GAAG SPES RASR VRAD EVRL QCQS GSGF LEGL FGCL RPVW TMIG KAYS TEHK QQQE DLWE VPFE EILD LQWV GSGA QGAV FLGR FHGE EVAV KKVR DLKE TDIK HLRK LKHP NIIT FK
Gene ID:7786
Uniprot:Q12852
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:93kDa/96kDa
Observed MW:120kDa
Synonyms:MAP3K12, DLK, MEKK12, MUK, ZPK, ZPKP1
Background:This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.
UniProt Protein Function:DLK: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
UniProt Protein Details:

Protein type:EC 2.7.11.25; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; MLK family; LZK subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; cytosol; membrane; plasma membrane

Molecular Function:protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: histone phosphorylation; JNK cascade; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation

NCBI Summary:This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]
UniProt Code:Q12852
NCBI GenInfo Identifier:116242624
NCBI Gene ID:7786
NCBI Accession:Q12852.2
UniProt Secondary Accession:Q12852,Q86VQ5, Q8WY25, B3KSS9, G3V1Y2,
UniProt Related Accession:Q12852
Molecular Weight:96,322 Da
NCBI Full Name:Mitogen-activated protein kinase kinase kinase 12
NCBI Synonym Full Names:mitogen-activated protein kinase kinase kinase 12
NCBI Official Symbol:MAP3K12  
NCBI Official Synonym Symbols:DLK; MUK; ZPK; ZPKP1; MEKK12  
NCBI Protein Information:mitogen-activated protein kinase kinase kinase 12
UniProt Protein Name:Mitogen-activated protein kinase kinase kinase 12
UniProt Synonym Protein Names:Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase
Protein Family:Mitogen-activated protein kinase kinase kinase
UniProt Gene Name:MAP3K12  
UniProt Entry Name:M3K12_HUMAN
Anti-MAP3K12 Polyclonal Antibody (CAB8464)Western blot analysis of extracts of various cell lines, using MAP3K12 antibody (CAB8464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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