Name: Anti-MAP3K1 Antibody (CAB18041)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18041
Availability: OutOfStock

Antibody Name:	Anti-MAP3K1 Antibody [KO Validated]
Antibody SKU:	CAB18041
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TGAG EFQG QLLG TIAF MAPE VLRG QQYG RSCD VWSV GCAI IEMA CAKP PWNA EKHS NHLA LIFK IASA TTAP SIPS HLSP GLRD VALR CLEL QPQD RPPS RELL KHPV FRTT W
Gene ID:	4214
Uniprot:	Q13233
Cellular Location:
Calculated MW:	164kDa
Observed MW:	164kDa

Anti-MAP3K1 Antibody [KO Validated] (CAB18041)

Western blot analysis of extracts from normal (control) and MAP3K1 knockout (KO) HeLa cells, using MAP3K1 antibody (CAB18041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.

Synonyms:

MAP3K1, MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6

Background:

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus.

UniProt Protein Function:	MEKK1: a protein kinase of the STE11 family. Phosphorylates and activates MKK4 and -7, which in turn activate JNK1, -2 and -3. Contains 1 RING-type zinc finger. The RING finger domain of MEKK1 exhibits E3 ubiquitin ligase activity toward c-Jun. c-Jun is downregulated in response to osmotic stress via ubiquitination-mediated degradation by the RING finger domain of MEKK1. Overexpressed/overactivated in multiple tumor types. The Mek1/2 inhibitor U0126 blocks export of influenza viral particles and has been suggested as an antiviral treatment. Single mutations at the same point of each gene also associated with cardiofaciocutaneous syndrome, (also linked with Braf and Kras mutations), displaying morphological, cardiac and mental defects. Inhibitors: U0126, CI-1040/PD184352, PD-0325901 (Phase I cancer), ARRY-142886 (Phase 1, cancer).
UniProt Protein Details:	Protein type:Ubiquitin ligase; Ligase; EC 2.7.11.25; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Ubiquitin conjugating system; STE group; STE11 family Chromosomal Location of Human Ortholog: 5q11.2 Cellular Component: cytosol Molecular Function:protein binding; zinc ion binding; MAP kinase kinase kinase activity; JUN kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: wound healing; activation of MAPKK activity; apoptosis; regulation of cell migration; protein amino acid phosphorylation; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; apoptotic mitochondrial changes; positive regulation of actin filament polymerization; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; actin cytoskeleton organization and biogenesis; toll-like receptor 4 signaling pathway Disease: 46,xy Sex Reversal 6
NCBI Summary:	The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]
UniProt Code:	Q13233
NCBI GenInfo Identifier:	218512139
NCBI Gene ID:	4214
NCBI Accession:	Q13233.4
UniProt Related Accession:	Q13233
Molecular Weight:	164,470 Da
NCBI Full Name:	Mitogen-activated protein kinase kinase kinase 1
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
NCBI Official Symbol:	MAP3K1
NCBI Official Synonym Symbols:	MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
NCBI Protein Information:	mitogen-activated protein kinase kinase kinase 1; MEK kinase 1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1
UniProt Protein Name:	Mitogen-activated protein kinase kinase kinase 1
UniProt Synonym Protein Names:	MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1
UniProt Gene Name:	MAP3K1
UniProt Entry Name:	M3K1_HUMAN