Antibody Name:	Anti-MANF Antibody [KO Validated]
Antibody SKU:	CAB13371
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-182 of human MANF (NP_006001.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	Jurkat, U-251MG, MCF7, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-182 of human MANF (NP_006001.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SRAL RPGD CEVC ISYL GRFY QDLK DRDV TFSP ATIE NELI KFCR EARG KENR LCYY IGAT DDAA TKII NEVS KPLA HHIP VEKI CEKL KKKD SQIC ELKY DKQI DLST VDLK KLRV KELK KILD DWGE TCKG CAEK SDYI RKIN ELMP KYAP KAAS AR
Gene ID:	7873
Uniprot:	P55145
Cellular Location:	Secreted
Calculated MW:	20kDa
Observed MW:	16kDa

Synonyms:

MANF, ARMET, ARP

Background:

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.

UniProt Protein Function:	ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: nucleus Disease: Pancreatic Cancer
NCBI Summary:	The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
UniProt Code:	P55145
NCBI GenInfo Identifier:	332278201
NCBI Gene ID:	7873
NCBI Accession:	P55145.3
UniProt Secondary Accession:	P55145,Q14CX4, Q86U67, Q96IS4,
UniProt Related Accession:	P55145
Molecular Weight:	20,700 Da
NCBI Full Name:	Mesencephalic astrocyte-derived neurotrophic factor
NCBI Synonym Full Names:	mesencephalic astrocyte derived neurotrophic factor
NCBI Official Symbol:	MANF
NCBI Official Synonym Symbols:	ARP; ARMET
NCBI Protein Information:	mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name:	Mesencephalic astrocyte-derived neurotrophic factor
UniProt Synonym Protein Names:	Arginine-rich protein; Protein ARMET
Protein Family:	MANF/CDNF-like protein
UniProt Gene Name:	MANF
UniProt Entry Name:	MANF_HUMAN

	Western blot analysis of extracts of various cell lines, using MANF Antibody (CAB13371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and MANF knockout (KO) 293T cells, using MANF antibody (CAB13371) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30S.
	Immunohistochemistry of paraffin-embedded human liver using MANF antibody (CAB13371) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MANF antibody (CAB13371) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate cancer using MANF antibody (CAB13371) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MANF Antibody (CAB7005)	Human ARMET / MANF ELISA Kit
Anti-MANF Antibody (CAB19923)[KO Validated]	Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit (HUES00359)
MANF Antibody (PACO02007)	Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit (HUES01703)
	Human Mesencephalic astrocyte-derived neurotrophic factor (MANF) ELISA Kit