Name: Anti-MAML1 Antibody (CAB17060)
Brand: Signal Transduction Antibodies 2
SKU: CAB17060
Availability: OutOfStock

Antibody Name:	Anti-MAML1 Antibody
Antibody SKU:	CAB17060
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MAML1 (NP_055572.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, mouse brain, rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MAML1 (NP_055572.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVLP TCPM AEFA LPRH SAVM ERLR RRIE LCRR HHST CEAR YEAV SPER LELE RQHT FALH QRCI QAKA KRAG KHRQ PPAA TAPA PAAP APRL DAAD GPEH GRPA THLH DTVK RNLD SATS PQNG DQQN GYGD LFPG HKKT RREA PLGV AISS NGLP PASP LGQS DKPS GADA LQSS GKHS LGLD SLNK KRLA DSSL HLNG GSNP SESF PLSL NKEL KQEP VEDL PCMI TGTV GSIS QSNL MPDL NLNE QEWK ELIE ELNR SVPD EDMK DLFN EDFE EKKD PESS GSAT QTPL AQDI NIKT
Gene ID:	9794
Uniprot:	Q92585
Cellular Location:
Calculated MW:	108kDa
Observed MW:	120kDa

Synonyms:

MAML1, Mam-1, Mam1

Background:

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

UniProt Protein Function:	MAML1: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. Belongs to the mastermind family.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 5q35 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:peptide antigen binding; protein binding; protein kinase binding; transcription coactivator activity Biological Process: activation of Notch receptor target transcription factor; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92585
NCBI GenInfo Identifier:	68565602
NCBI Gene ID:	9794
NCBI Accession:	Q92585.3
UniProt Secondary Accession:	Q92585,Q9NZ12,
UniProt Related Accession:	Q92585
Molecular Weight:	108,054 Da
NCBI Full Name:	Mastermind-like protein 1
NCBI Synonym Full Names:	mastermind like transcriptional coactivator 1
NCBI Official Symbol:	MAML1
NCBI Official Synonym Symbols:	Mam1; Mam-1
NCBI Protein Information:	mastermind-like protein 1
UniProt Protein Name:	Mastermind-like protein 1
UniProt Gene Name:	MAML1
UniProt Entry Name:	MAML1_HUMAN

Western blot analysis of extracts of various cell lines, using MAML1 antibody (CAB17060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

ELISA Kits

Human MAML1 (Mastermind-like protein 1) ELISA Kit (HUFI03513)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)