Antibody Name:	Anti-MAGEA1 Antibody
Antibody SKU:	CAB5470
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	22Rv1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLE QRSL HCKP EEAL EAQQ EALG LVCV QAAT SSSS PLVL GTLE EVPT AGST DPPQ SPQG ASAF PTTI NFTR QRQP SEGS SSRE EEGP STSC ILES LFRA VITK KVAD LVGF LLLK YRAR EPVT KAEM LESV IKNY KHCF PEIF GKAS ESLQ LVFG IDVK EADP TGHS YVLV TCLG LSYD GLLG DNQI MPKT GFLI IVLV MIAM EGGH APEE EIWE ELSV MEVY DGRE HSAY GEPR KLLT QDLV QEKY LEYR QVPD SDPA RYEF LWGP RALA ETSY VKVL EYVI KVSA RVRF FFPS LREA ALRE EEEG V
Gene ID:	4100
Uniprot:	P43355
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	34kDa
Observed MW:	45kDa

Synonyms:

MAGEA1, CT1.1, MAGE1

Background:

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

UniProt Protein Function:	MAGE-A1: a tumor antigen expressed in a variety of tumor cells and in the testis. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
UniProt Protein Details:	Protein type:Cell surface Chromosomal Location of Human Ortholog: Xq28 Cellular Component: cytoplasm; plasma membrane; nucleus Molecular Function:protein binding; histone deacetylase binding Biological Process: transcription, DNA-dependent; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
UniProt Code:	P43355
NCBI GenInfo Identifier:	1170855
NCBI Gene ID:	4100
NCBI Accession:	P43355.1
UniProt Secondary Accession:	P43355,O00346, B2RC81,
UniProt Related Accession:	P43355,AAB33086
Molecular Weight:	309
NCBI Full Name:	Melanoma-associated antigen 1
NCBI Synonym Full Names:	melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
NCBI Official Symbol:	MAGEA1
NCBI Official Synonym Symbols:	CT1.1; MAGE1
NCBI Protein Information:	melanoma-associated antigen 1; antigen MZ2-E; MAGE-1 antigen; melanoma antigen MAGE-1; cancer/testis antigen 1.1; melanoma antigen family A 1; melanoma-associated antigen MZ2-E; cancer/testis antigen family 1, member 1
UniProt Protein Name:	Melanoma-associated antigen 1
UniProt Synonym Protein Names:	Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigen
Protein Family:	Melanoma-associated antigen
UniProt Gene Name:	MAGEA1
UniProt Entry Name:	MAGA1_HUMAN

	Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (CAB5470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody (CAB5470) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody (CAB5470) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody (CAB5470) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using MAGEA1 antibody (CAB5470). Blue: DAPI for nuclear staining.