Epigenetics & Nuclear Signaling Antibodies 2

Anti-MAFG Antibody (CAB16056)

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SKU:
CAB16056
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MAFG Antibody
Antibody SKU:CAB16056
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human MAFG (NP_002350.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, A-549, HT-29, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human MAFG (NP_002350.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTTP NKGN KALK VKRE PGEN GTSL TDEE LVTM SVRE LNQH LRGL SKEE IVQL KQRR RTLK NRGY AASC RVKR VTQK EELE KQKA ELQQ EVEK LASE NASM KLEL DALR SKYE ALQT FART VARS PVAP ARGP LAAG LGPL VPGK VAAT SVIT IVKS KTDA RS
Gene ID:4097
Uniprot:O15525
Cellular Location:Nucleus
Calculated MW:17kDa
Observed MW:18kDa
Synonyms:MAFG, hMAF
Background:Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).
UniProt Protein Function:MAFG: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). Belongs to the bZIP family. Maf subfamily.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: blood coagulation; regulation of transcription from RNA polymerase II promoter

NCBI Summary:Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]
UniProt Code:O15525
NCBI GenInfo Identifier:6685623
NCBI Gene ID:4097
NCBI Accession:O15525.1
UniProt Related Accession:O15525
Molecular Weight:17,850 Da
NCBI Full Name:Transcription factor MafG
NCBI Synonym Full Names:MAF bZIP transcription factor G
NCBI Official Symbol:MAFG  
NCBI Official Synonym Symbols:hMAF  
NCBI Protein Information:transcription factor MafG
UniProt Protein Name:Transcription factor MafG
UniProt Synonym Protein Names:V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF
Protein Family:Transcription factor
UniProt Gene Name:MAFG  
UniProt Entry Name:MAFG_HUMAN
Anti-MAFG Antibody (CAB16056)Western blot analysis of extracts of various cell lines, using MAFG antibody (CAB16056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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