Metabolism Antibodies 1

Anti-MAFF Antibody (CAB12920)

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SKU:
CAB12920
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-MAFF Antibody
Antibody SKU:CAB12920
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 75-164 of human MAFF (NP_036455.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HT-29
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 75-164 of human MAFF (NP_036455.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QKEE LQKQ KSEL EREV DKLA RENA AMRL ELDA LRGK CEAL QGFA RSVA AARG PATL VAPA SVIT IVKS TPGS GSGP AHGP DPAH GPAS CS
Gene ID:23764
Uniprot:Q9ULX9
Cellular Location:Nucleus
Calculated MW:14kDa/17kDa
Observed MW:18kDa
Synonyms:MAFF, U-MAF, hMafF
Background:The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:MAFF: Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: mitochondrion; nucleoplasm; nucleus

Biological Process: blood coagulation; parturition; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
UniProt Code:Q9ULX9
NCBI GenInfo Identifier:21542145
NCBI Gene ID:23764
NCBI Accession:Q9ULX9.2
UniProt Secondary Accession:Q9ULX9,Q9Y525, B4DV49,
UniProt Related Accession:Q9ULX9
Molecular Weight:14,569 Da
NCBI Full Name:Transcription factor MafF
NCBI Synonym Full Names:MAF bZIP transcription factor F
NCBI Official Symbol:MAFF  
NCBI Official Synonym Symbols:U-MAF; hMafF  
NCBI Protein Information:transcription factor MafF
UniProt Protein Name:Transcription factor MafF
UniProt Synonym Protein Names:U-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F
Protein Family:MaFF-interacting protein
UniProt Gene Name:MAFF  
UniProt Entry Name:MAFF_HUMAN
Anti-MAFF Antibody (CAB12920)Western blot analysis of extracts of HT-29 cells, using MAFF antibody (CAB12920) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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