Epigenetics & Nuclear Signaling Antibodies 1

Anti-MAFB Antibody (CAB10077)

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SKU:
CAB10077
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MAFB Antibody
Antibody SKU:CAB10077
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 184-323 of human MAFB (NP_005452.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 184-323 of human MAFB (NP_005452.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PTSH PGPG PHAT ASAT AAGG NGSV EDRF SDDQ LVSM SVRE LNRH LRGF TKDE VIRL KQKR RTLK NRGY AQSC RYKR VQQK HHLE NEKT QLIQ QVEQ LKQE VSRL ARER DAYK VKCE KLAN SGFR EAGS TSDS PSSP EFFL
Gene ID:9935
Uniprot:Q9Y5Q3
Cellular Location:Nucleus
Calculated MW:35kDa
Observed MW:50kDa
Synonyms:MAFB, DURS3, KRML, MCTO
Background:The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.
UniProt Protein Function:MAFB: Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO). MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients. Belongs to the bZIP family. Maf subfamily.
UniProt Protein Details:

Protein type:DNA-binding; Tumor suppressor; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 20q12

Molecular Function:protein binding; transcription factor binding

Biological Process: negative regulation of erythrocyte differentiation; negative regulation of osteoclast differentiation; nervous system development; regulation of transcription from RNA polymerase II promoter; sensory organ development

Disease: Multicentric Carpotarsal Osteolysis Syndrome

NCBI Summary:The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y5Q3
NCBI GenInfo Identifier:21759268
NCBI Gene ID:9935
NCBI Accession:Q9Y5Q3.2
UniProt Secondary Accession:Q9Y5Q3,Q9H1F1, B3KNE1,
UniProt Related Accession:Q9Y5Q3
Molecular Weight:35,792 Da
NCBI Full Name:Transcription factor MafB
NCBI Synonym Full Names:MAF bZIP transcription factor B
NCBI Official Symbol:MAFB  
NCBI Official Synonym Symbols:KRML; MCTO  
NCBI Protein Information:transcription factor MafB
UniProt Protein Name:Transcription factor MafB
UniProt Synonym Protein Names:V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Protein Family:Transcription factor
UniProt Gene Name:MAFB  
UniProt Entry Name:MAFB_HUMAN
Anti-MAFB Antibody (CAB10077)Western blot analysis of extracts of various cell lines, using MAFB antibody (CAB10077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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