Epigenetics & Nuclear Signaling Antibodies 2
Anti-MAF1 Antibody (CAB15531)
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- SKU:
- CAB15531
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MAF1 Antibody |
| Antibody SKU: | CAB15531 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MAF1 (NP_115648.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, HeLa, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MAF1 (NP_115648.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKLL ENSS FEAI NSQL TVET GDAH IIGR IESY SCKM AGDD KHMF KQFC QEGQ PHVL EALS PPQT SGLS PSRL SKSQ GGEE EGPL SDKC SRKT LFYL IATL NESF RPDY DFST ARSH EFSR EPSL SWVV NAVN CSLF SAVR EDFK DLKP QLWN AVDE EICL AECD IYSY NPDL DSDP FGED GSLW SFNY FFYN KRLK RIVF FSCR SISG STYT PSEA GNEL DMEL GEEE VEEE SRSG GSGA EETS TMEE DRVP VICI |
| Gene ID: | 84232 |
| Uniprot: | Q9H063 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 28kDa |
| Observed MW: | 37kDa |
| Synonyms: | MAF1, MAF1 homolog |
| Background: | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. |
| UniProt Protein Function: | MAF1: Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. Belongs to the MAF1 family. |
| UniProt Protein Details: | Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; axon; dendrite; cytoplasm; plasma membrane; nucleolus; intracellular; nucleus; cytosol Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase III promoter |
| UniProt Code: | Q9H063 |
| NCBI GenInfo Identifier: | 49065352 |
| NCBI Gene ID: | |
| NCBI Accession: | CAG38494.1 |
| UniProt Secondary Accession: | Q9H063,D3DWL4, |
| Molecular Weight: | 28,771 Da |
| NCBI Full Name: | MAF1 |
| UniProt Protein Name: | Repressor of RNA polymerase III transcription MAF1 homolog |
| Protein Family: | Maf-like protein |
| UniProt Gene Name: | MAF1 |
| UniProt Entry Name: | MAF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAF1 antibody (CAB15531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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