Name: Anti-MAD2L2 Antibody (CAB12559)
Brand: Cell Cycle Antibodies 1
SKU: CAB12559
Availability: OutOfStock

Antibody Name:	Anti-MAD2L2 Antibody
Antibody SKU:	CAB12559
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2L2 (NP_006332.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, A375, K-562, SW480, HeLa, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2L2 (NP_006332.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTL TRQD LNFG QVVA DVLC EFLE VAVH LILY VREV YPVG IFQK RKKY NVPV QMSC HPEL NQYI QDTL HCVK PLLE KNDV EKVV VVIL DKEH RPVE KFVF EITQ PPLL SISS DSLL SHVE QLLR AFIL KISV CDAV LDHN PPGC TFTV LVHT REAA TRNM EKIQ VIKD FPWI LADE QDVH MHDP RLIP LKTM TSDI LKMQ LYVE ERAH KGS
Gene ID:	10459
Uniprot:	Q9UI95
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:	24kDa
Observed MW:	24kDa

Synonyms:	MAD2L2, FANCV, MAD2B, POLZ2, REV7
Background:	The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins.

UniProt Protein Function:	MAD2L2: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: anaphase-promoting complex; cytosol; nucleolus; nucleoplasm; nucleus; spindle; zeta DNA polymerase complex Molecular Function:JUN kinase binding; protein binding Biological Process: actin filament organization; DNA damage response, signal transduction resulting in transcription; double-strand break repair; error-prone postreplication DNA repair; mitotic cell cycle spindle assembly checkpoint; negative regulation of protein catabolic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription, DNA-dependent; regulation of cell growth Disease: Fanconi Anemia, Complementation Group V
NCBI Summary:	The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UI95
NCBI GenInfo Identifier:	12643889
NCBI Gene ID:	10459
NCBI Accession:	Q9UI95.2
UniProt Secondary Accession:	Q9UI95,Q5TGW7, Q9UNA7, Q9Y6I6, B3KNE3,
UniProt Related Accession:	Q9UI95
Molecular Weight:	24kDa
NCBI Full Name:	Mitotic spindle assembly checkpoint protein MAD2B
NCBI Synonym Full Names:	mitotic arrest deficient 2 like 2
NCBI Official Symbol:	MAD2L2
NCBI Official Synonym Symbols:	REV7; FANCV; MAD2B; POLZ2
NCBI Protein Information:	mitotic spindle assembly checkpoint protein MAD2B
UniProt Protein Name:	Mitotic spindle assembly checkpoint protein MAD2B
UniProt Synonym Protein Names:	Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7
Protein Family:	Mitotic spindle assembly checkpoint protein
UniProt Gene Name:	MAD2L2

	Western blot analysis of extracts of various cell lines, using MAD2L2 antibody (CAB12559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of H9C2 cells using MAD2L2 antibody (CAB12559) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using MAD2L2 antibody (CAB12559) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using MAD2L2 antibody (CAB12559) at dilution of 1:100. Blue: DAPI for nuclear staining.