Cell Cycle Antibodies 1
Anti-MAD2L1 Antibody (CAB1699)
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- SKU:
- CAB1699
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MAD2L1 Antibody |
| Antibody SKU: | CAB1699 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MAD2L1 (NP_002349.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | MCF7, SW480, K-562, Jurkat, 293T, 22Rv1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MAD2L1 (NP_002349.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALQ LSRE QGIT LRGS AEIV AEFF SFGI NSIL YQRG IYPS ETFT RVQK YGLT LLVT TDLE LIKY LNNV VEQL KDWL YKCS VQKL VVVI SNIE SGEV LERW QFDI ECDK TAKD DSAP REKS QKAI QDEI RSVI RQIT ATVT FLPL LEVS CSFD LLIY TDKD LVVP EKWE ESGP QFIT NSEE VRLR SFTT TIHK VNSM VAYK IPVN D |
| Gene ID: | 4085 |
| Uniprot: | Q13257 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle pole |
| Calculated MW: | 10kDa/23kDa |
| Observed MW: | 24kDa |
| Synonyms: | MAD2L1, HSMAD2, MAD2 |
| Background: | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. |
| UniProt Protein Function: | MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 4q27 Cellular Component: cytosol; kinetochore; nuclear pore; nucleus; perinuclear region of cytoplasm Molecular Function:identical protein binding; protein binding; protein homodimerization activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; sister chromatid cohesion |
| NCBI Summary: | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13257 |
| NCBI GenInfo Identifier: | 12230256 |
| NCBI Gene ID: | 4085 |
| NCBI Accession: | Q13257.1 |
| UniProt Secondary Accession: | Q13257,Q53F56, Q548X9, Q6IRW7, Q8IZX3, |
| UniProt Related Accession: | Q13257 |
| Molecular Weight: | 10,335 Da |
| NCBI Full Name: | Mitotic spindle assembly checkpoint protein MAD2A |
| NCBI Synonym Full Names: | MAD2 mitotic arrest deficient-like 1 (yeast) |
| NCBI Official Symbol: | MAD2L1 |
| NCBI Official Synonym Symbols: | MAD2; HSMAD2 |
| NCBI Protein Information: | mitotic spindle assembly checkpoint protein MAD2A |
| UniProt Protein Name: | Mitotic spindle assembly checkpoint protein MAD2A |
| UniProt Synonym Protein Names: | Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1 |
| Protein Family: | MAD2L1-binding protein |
| UniProt Gene Name: | MAD2L1 |
| UniProt Entry Name: | MD2L1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAD2L1 antibody (CAB1699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using MAD2L1 antibody (CAB1699). Blue: DAPI for nuclear staining. |
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