Cell Cycle Antibodies 1

Anti-MAD1L1 Antibody (CAB1153)

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SKU:
CAB1153
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MAD1L1 Antibody
Antibody SKU:CAB1153
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, HepG2, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RGLE KARQ QLQE ELRQ VSGQ LLEE RKKR ETHE ALAR RLQK RVLL LTKE RDGM RAIL GSYD SELT PAEY SPQL TRRM REAE DMVQ KVHS HSAE MEAQ LSQA LEEL GGQK QRAD MLEM ELKM LKSQ SSSA EQSF LFSR EEAD TLRL KVEE LEGE RSRL EEEK RMLE AQLE RRAL QGDY DQSR TKVL HMSL NPTS VARQ RLRE DHSQ LQAE CERL RGLL RAME RGGT VPAD LEAA AASL PSSK EVAE LKKQ VESA ELKN QRLK EVFQ TKIQ EFRK ACYT LTGY QIDI TTEN QYRL TSLY AEHP GDCL IFKA TSPS GSKM QLLE TEFS HTVG ELIE VHLR RQDS I
Gene ID:8379
Uniprot:Q9Y6D9
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Calculated MW:72kDa/83kDa
Observed MW:95kDa
Synonyms:MAD1L1, MAD1, PIG9, TP53I9, TXBP181
Background:MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
UniProt Protein Details:

Protein type:Cell cycle regulation; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: kinetochore; centrosome; cytoplasm; spindle; nuclear pore; nucleus; cytosol; actin cytoskeleton

Molecular Function:protein binding

Biological Process: mitosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

Disease: Prostate Cancer

NCBI Summary:MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y6D9
NCBI GenInfo Identifier:52783153
NCBI Gene ID:8379
NCBI Accession:Q9Y6D9.2
UniProt Secondary Accession:Q9Y6D9,Q13312, Q75MI0, Q86UM4, Q9UNH0, B3KR41,
UniProt Related Accession:Q9Y6D9
Molecular Weight:718
NCBI Full Name:Mitotic spindle assembly checkpoint protein MAD1
NCBI Synonym Full Names:MAD1 mitotic arrest deficient-like 1 (yeast)
NCBI Official Symbol:MAD1L1  
NCBI Official Synonym Symbols:MAD1; PIG9; TP53I9; TXBP181  
NCBI Protein Information:mitotic spindle assembly checkpoint protein MAD1; MAD1-like protein 1; tax-binding protein 181; tumor protein p53 inducible protein 9; mitotic checkpoint MAD1 protein homolog; mitotic arrest deficient 1-like protein 1; mitotic-arrest deficient 1, yeast, homolog-like 1
UniProt Protein Name:Mitotic spindle assembly checkpoint protein MAD1
UniProt Synonym Protein Names:Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181
Protein Family:Mitotic spindle assembly checkpoint protein
UniProt Gene Name:MAD1L1  
UniProt Entry Name:MD1L1_HUMAN
Anti-MAD1L1 Antibody (CAB1153)Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (CAB1153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-MAD1L1 Antibody (CAB1153)Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (CAB1153) at dilution of 1:100 (40x lens).
Anti-MAD1L1 Antibody (CAB1153)Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (CAB1153) at dilution of 1:100 (40x lens).
Anti-MAD1L1 Antibody (CAB1153)Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (CAB1153).
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