Antibody Name:	Anti-macroH2A.1 Antibody [KO Validated]
Antibody SKU:	CAB7045
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Jurkat, 293T, HeLa, Mouse liver, Mouse spleen, Mouse thymus, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLEA IITP PPAK KAKS PSQK KPVS KKAG GKKG ARKS KKKQ GEVS KAAS ADST TEGT PADG FTVL STKS LFLG QKLN LIHS EISN LAGF EVEA IINP TNAD IDLK DDLG NTLE KKGG KEFV EAVL ELRK KNGP LEVA GAAV SAGH GLPA KFVI HCNS PVWG ADKC EELL EKTV KNCL ALAD DKKL KSIA FPSI GSGR NGFP KQTA AQLI LKAI SSYF VSTM SSSI KTVY FVLF DSES IGIY VQEM AKLD AN
Gene ID:	9555
Uniprot:	O75367
Cellular Location:	Chromosome, Nucleus
Calculated MW:	39kDa
Observed MW:	40kDa

Synonyms:

H2AFY, H2A.y, H2A/y, H2AF12M, MACROH2A1.1, mH2A1, macroH2A1.2

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	H2AFY: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: nucleoplasm; centric heterochromatin; sex chromatin; ESC/E(Z) complex; nuclear chromatin; nucleolus; Barr body; condensed chromosome; nucleosome; nucleus Molecular Function:rDNA binding; protein serine/threonine kinase inhibitor activity; protein binding; DNA binding; double-stranded methylated DNA binding; chromatin DNA binding; protein heterodimerization activity; protein kinase binding Biological Process: nucleosome assembly; negative regulation of gene expression, epigenetic; dosage compensation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of cell growth; negative regulation of histone phosphorylation
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O75367
NCBI GenInfo Identifier:	90110023
NCBI Gene ID:	9555
NCBI Accession:	O75367.4
UniProt Secondary Accession:	O75367,O75377, Q503A8, Q7Z5E3, Q96D41, Q9H8P3, Q9UP96
UniProt Related Accession:	O75367
Molecular Weight:	372
NCBI Full Name:	Core histone macro-H2A.1
NCBI Synonym Full Names:	H2A histone family, member Y
NCBI Official Symbol:	H2AFY
NCBI Official Synonym Symbols:	H2A.y; H2A/y; H2AFJ; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2
NCBI Protein Information:	core histone macro-H2A.1; histone H2A.y; histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; medulloblastoma antigen MU-MB-50.205
UniProt Protein Name:	Core histone macro-H2A.1
UniProt Synonym Protein Names:	Histone H2A.y; H2A/y; Medulloblastoma antigen MU-MB-50.205
Protein Family:	Core histone macro
UniProt Gene Name:	H2AFY
UniProt Entry Name:	H2AY_HUMAN

	Western blot analysis of extracts of various cell lines, using H2AFY antibody (CAB7045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and macroH2A.1 Rabbit pAb knockout (KO) 293T cells, using macroH2A.1 Rabbit pAb antibody (CAB7045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human kidney using macroH2A.1 antibody (CAB7045) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using macroH2A.1 antibody (CAB7045) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using macroH2A.1 antibody (CAB7045) at dilution of 1:100 (40x lens).