Name: Anti-M6PR Antibody (CAB19907)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19907
Availability: OutOfStock

Antibody Name:	Anti-M6PR Antibody [KO Validated]
Antibody SKU:	CAB19907
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human M6PR.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant protein of human M6PR.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4074
Uniprot:	P20645
Cellular Location:	Lysosome membrane, Single-pass type I membrane protein
Calculated MW:	30kDa
Observed MW:	31KDa

Anti-M6PR Antibody [KO Validated] (CAB19907)

Western blot analysis of extracts from normal (control) and M6PR knockout (KO) 293T cells, using M6PR antibody (CAB19907) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

M6PR, CD-M6PR, CD-MPR, MPR 46, MPR-46, MPR46, SMPR

Background:

This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.

UniProt Protein Function:	M6PR: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 12p13 Cellular Component: endosome; integral to plasma membrane; membrane; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network membrane; transport vesicle Molecular Function:transmembrane receptor activity Biological Process: endosome to lysosome transport; receptor-mediated endocytosis
NCBI Summary:	This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, May 2011]
UniProt Code:	P20645
NCBI GenInfo Identifier:	127293
NCBI Gene ID:	4074
NCBI Accession:	P20645.1
UniProt Secondary Accession:	P20645,A8K528, D3DUV5,
UniProt Related Accession:	P20645
Molecular Weight:	30,993 Da
NCBI Full Name:	Cation-dependent mannose-6-phosphate receptor
NCBI Synonym Full Names:	mannose-6-phosphate receptor, cation dependent
NCBI Official Symbol:	M6PR
NCBI Official Synonym Symbols:	SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
NCBI Protein Information:	cation-dependent mannose-6-phosphate receptor
UniProt Protein Name:	Cation-dependent mannose-6-phosphate receptor
UniProt Synonym Protein Names:	46 kDa mannose 6-phosphate receptor; MPR 46
Protein Family:	Cation-dependent mannose-6-phosphate receptor
UniProt Gene Name:	M6PR
UniProt Entry Name:	MPRD_HUMAN