Anti-LysRS/KARS Antibody (CAB8648)

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SKU:
CAB8648
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CMTRIB
Synonyms:
DFNB89
Synonyms:
KARS1
Synonyms:
KARS2
Synonyms:
KRS
Research Area:
Immunology

Description

Product Name:LysRS/KARS Rabbit mAb
Product Code:CAB8648
Size:20uL, 50uL, 100uL
Synonyms:CMTRIB, DFNB89, KARS1, KARS2, KRS
Applications:WB, IHC, IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human LysRS/KARS
Applications:WB, IHC, IF
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, C6, Mouse testis, Rat testis
Immunogen:A synthesized peptide derived from human LysRS/KARS
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3735
Uniprot:Q15046
Cellular Location:Cell membrane, Cytoplasm, Mitochondrion, Nucleus, Peripheral membrane protein, Secreted
Calculated MW:73kDa
Observed MW:75kDa
UniProt Protein Function:KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; EC 6.1.1.6; Translation; Ligase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; mitochondrial matrix; mitochondrion

Molecular Function:lysine-tRNA ligase activity; protein binding

Biological Process: lysyl-tRNA aminoacylation; tRNA aminoacylation for protein translation

Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89

NCBI Summary:Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q15046
NCBI GenInfo Identifier:20178333
NCBI Gene ID:3735
NCBI Accession:Q15046.3
UniProt Secondary Accession:Q15046,O14946, Q96J25, Q9HB23, A8MSK1, D3DUK4,
UniProt Related Accession:Q15046
Molecular Weight:71,497 Da
NCBI Full Name:Lysine--tRNA ligase
NCBI Synonym Full Names:lysyl-tRNA synthetase
NCBI Official Symbol:KARS  
NCBI Official Synonym Symbols:KRS; KARS1; KARS2; CMTRIB; DFNB89  
NCBI Protein Information:lysine--tRNA ligase
UniProt Protein Name:Lysine--tRNA ligase
UniProt Synonym Protein Names:Lysyl-tRNA synthetase; LysRS
UniProt Gene Name:KARS  
UniProt Entry Name:SYK_HUMAN
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