Description
| Product Name: | LysRS/KARS Rabbit mAb |
| Product Code: | CAB8648 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | CMTRIB, DFNB89, KARS1, KARS2, KRS |
| Applications: | WB, IHC, IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human LysRS/KARS |
| Applications: | WB, IHC, IF |
| Recommended Dilutions: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, C6, Mouse testis, Rat testis |
| Immunogen: | A synthesized peptide derived from human LysRS/KARS |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3735 |
| Uniprot: | Q15046 |
| Cellular Location: | Cell membrane, Cytoplasm, Mitochondrion, Nucleus, Peripheral membrane protein, Secreted |
| Calculated MW: | 73kDa |
| Observed MW: | 75kDa |
| UniProt Protein Function: | KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 6.1.1.6; Translation; Ligase Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; mitochondrial matrix; mitochondrion Molecular Function:lysine-tRNA ligase activity; protein binding Biological Process: lysyl-tRNA aminoacylation; tRNA aminoacylation for protein translation Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89 |
| NCBI Summary: | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15046 |
| NCBI GenInfo Identifier: | 20178333 |
| NCBI Gene ID: | 3735 |
| NCBI Accession: | Q15046.3 |
| UniProt Secondary Accession: | Q15046,O14946, Q96J25, Q9HB23, A8MSK1, D3DUK4, |
| UniProt Related Accession: | Q15046 |
| Molecular Weight: | 71,497 Da |
| NCBI Full Name: | Lysine--tRNA ligase |
| NCBI Synonym Full Names: | lysyl-tRNA synthetase |
| NCBI Official Symbol: | KARS |
| NCBI Official Synonym Symbols: | KRS; KARS1; KARS2; CMTRIB; DFNB89 |
| NCBI Protein Information: | lysine--tRNA ligase |
| UniProt Protein Name: | Lysine--tRNA ligase |
| UniProt Synonym Protein Names: | Lysyl-tRNA synthetase; LysRS |
| UniProt Gene Name: | KARS |
| UniProt Entry Name: | SYK_HUMAN |
