Immunology Antibodies 1

Anti-LY9 Antibody (CAB10032)

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SKU:
CAB10032
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-LY9 Antibody
Antibody SKU:CAB10032
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-360 of human LY9 (NP_001248386.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse
Positive Samples:K-562, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-360 of human LY9 (NP_001248386.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TMKS VKVS ENFS CNIT LMCS VKGA EKSV LYSW TPRE PHAS ESNG GSIL TVSR TPCD PDLP YICT AQNP VSQR SSLP VHVG QFCT DPGA SRGG TTGE TVVG VLGE PVTL PLAL PACR DTEK VVWL FNTS IISK EREE AATA DPLI KSRD PYKN RVWV SSQD CSLK ISQL KIED AGPY HAYV CSEA SSVT SMTH VTLL IYRP E
Gene ID:4063
Uniprot:Q9HBG7
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:57kDa/70kDa/71kDa/72kDa
Observed MW:90kDa
Synonyms:LY9, CD229, SLAMF3, hly9, mLY9
Background:
UniProt Protein Function:CD229: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane

Molecular Function:receptor activity

Biological Process: cell adhesion

NCBI Summary:LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9HBG7
NCBI GenInfo Identifier:75991709
NCBI Gene ID:4063
NCBI Accession:NP_001028839.1
UniProt Secondary Accession:Q9HBG7,Q14775, Q5VYI3, Q6P2J4, Q9H4N5, Q9NQ24, A8K7N3
UniProt Related Accession:Q9HBG7
Molecular Weight:
NCBI Full Name:T-lymphocyte surface antigen Ly-9 isoform b
NCBI Synonym Full Names:lymphocyte antigen 9
NCBI Official Symbol:LY9  
NCBI Official Synonym Symbols:hly9; mLY9; CD229; SLAMF3  
NCBI Protein Information:T-lymphocyte surface antigen Ly-9
UniProt Protein Name:T-lymphocyte surface antigen Ly-9
UniProt Synonym Protein Names:Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD_antigen: CD229
Protein Family:T-lymphocyte surface antigen
UniProt Gene Name:LY9  
UniProt Entry Name:LY9_HUMAN
Anti-LY9 Antibody (CAB10032)Western blot analysis of extracts of various cell lines, using LY9 antibody (CAB10032) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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