Epigenetics & Nuclear Signaling Antibodies 1
Anti-LSM3 Antibody (CAB13189)
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- SKU:
- CAB13189
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-LSM3 Antibody |
| Antibody SKU: | CAB13189 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human LSM3 (NP_055278.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human LSM3 (NP_055278.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADD VDQQ QTTN TVEE PLDL IRLS LDER IYVK MRND RELR GRLH AYDQ HLNM ILGD VEET VTTI EIDE ETYE EIYK STKR NIPM LFVR GDGV VLVA PPLR VG |
| Gene ID: | 27258 |
| Uniprot: | P62310 |
| Cellular Location: | Nucleus |
| Calculated MW: | 11kDa |
| Observed MW: | 12kDa |
| Synonyms: | LSM3, SMX4, USS2, YLR438C |
| Background: |
| UniProt Protein Function: | LSM3: Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 3p25.1 Cellular Component: cytosol; nucleus Molecular Function:protein binding Biological Process: nuclear mRNA splicing, via spliceosome; gene expression; mRNA processing; mRNA catabolic process, deadenylation-dependent decay |
| NCBI Summary: | Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004] |
| UniProt Code: | P62310 |
| NCBI GenInfo Identifier: | 7657315 |
| NCBI Gene ID: | 27258 |
| NCBI Accession: | NP_055278.1 |
| UniProt Secondary Accession: | P62310,Q6IAH0, Q9Y4Z1, |
| UniProt Related Accession: | P62310 |
| Molecular Weight: | 11,845 Da |
| NCBI Full Name: | U6 snRNA-associated Sm-like protein LSm3 |
| NCBI Synonym Full Names: | LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) |
| NCBI Official Symbol: | LSM3 |
| NCBI Official Synonym Symbols: | SMX4; USS2; YLR438C |
| NCBI Protein Information: | U6 snRNA-associated Sm-like protein LSm3 |
| UniProt Protein Name: | U6 snRNA-associated Sm-like protein LSm3 |
| Protein Family: | U6 snRNA-associated Sm-like protein |
| UniProt Gene Name: | LSM3 |
| UniProt Entry Name: | LSM3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LSM3 antibody (CAB13189) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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