Epigenetics & Nuclear Signaling Antibodies 4

Anti-LSM2 Antibody (CAB7988)

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SKU:
CAB7988
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:LSM2 Rabbit Polyclonal Antibody
Antibody SKU:CAB7988
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human LSM2 (NP_067000.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:SW620, SKOV3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human LSM2 (NP_067000.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLFY SFFK SLVG KDVV VELK NDLS ICGT LHSV DQYL NIKL TDIS VTDP EKYP HMLS VKNC FIRG SVVR YVQL PADE VDTQ LLQD AARK EALQ QKQ
Gene ID:57819
Uniprot:Q9Y333
Cellular Location:Nucleus
Calculated MW:10kDa
Observed MW:13kDa
Synonyms:LSM2, C6orf28, G7B, YBL026W, snRNP
Background:This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19.
UniProt Protein Function:LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.
UniProt Protein Details:

Protein type:RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytosol; nucleoplasm; U4/U6 x U5 tri-snRNP complex

Molecular Function:protein binding; Ral GTPase binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome

NCBI Summary:This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]
UniProt Code:Q9Y333
NCBI GenInfo Identifier:10720079
NCBI Gene ID:57819
NCBI Accession:Q9Y333.1
UniProt Secondary Accession:Q9Y333,Q6FGG1,
UniProt Related Accession:Q9Y333
Molecular Weight:10,835 Da
NCBI Full Name:U6 snRNA-associated Sm-like protein LSm2
NCBI Synonym Full Names:LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
NCBI Official Symbol:LSM2  
NCBI Official Synonym Symbols:G7B; snRNP; C6orf28; YBL026W  
NCBI Protein Information:U6 snRNA-associated Sm-like protein LSm2
UniProt Protein Name:U6 snRNA-associated Sm-like protein LSm2
UniProt Synonym Protein Names:Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5
Protein Family:Sm-like protein
UniProt Gene Name:LSM2  
UniProt Entry Name:LSM2_HUMAN
Anti-LSM2 Polyclonal Antibody (CAB7988)Western blot analysis of extracts of various cell lines, using LSM2 antibody (CAB7988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-LSM2 Polyclonal Antibody (CAB7988)Immunohistochemistry of paraffin-embedded human gastric cancer using LSM2 antibody (CAB7988) at dilution of 1:100 (40x lens).
Anti-LSM2 Polyclonal Antibody (CAB7988)Immunofluorescence analysis of U2OS cells using LSM2 antibody (CAB7988) at dilution of 1:100. Blue: DAPI for nuclear staining.
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