Epigenetics & Nuclear Signaling Antibodies 2

Anti-Lrwd1 Antibody (CAB15576)

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SKU:
CAB15576
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Lrwd1 Antibody
Antibody SKU:CAB15576
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Lrwd1 (NP_690852.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, HepG2, Jurkat, Mouse testis, Mouse thymus, Rat testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Lrwd1 (NP_690852.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASPS AQVE GSPV AGSD GSQP AVKL EPLH FLQC HSKN NSPQ DLET QLWA CAFE PAWE EGAT SQTV ATCG GEAV CVID CQTG IVLH KYKA PGEE FFSV AWTA L
Gene ID:222229
Uniprot:Q9UFC0
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, microtubule organizing center, telomere
Calculated MW:70kDa
Observed MW:65kDa
Synonyms:LRWD1, CENP-33, ORCA, Lrwd1
Background:The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:LRWD1: Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors. Belongs to the LRWD1 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: centric heterochromatin; cytoplasm; microtubule organizing center; nuclear origin of replication recognition complex; nucleus

Molecular Function:protein binding; methyl-CpG binding; chromatin binding; methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication initiation; chromatin modification

UniProt Code:Q9UFC0
NCBI GenInfo Identifier:23097240
NCBI Gene ID:222229
NCBI Accession:NP_690852.1
UniProt Secondary Accession:Q9UFC0,Q8N0T9, Q8WV43, Q96GJ2, A8K4K2, B2R9G2,
UniProt Related Accession:Q9UFC0
Molecular Weight:70,861 Da
NCBI Full Name:leucine-rich repeat and WD repeat-containing protein 1
NCBI Synonym Full Names:leucine-rich repeats and WD repeat domain containing 1
NCBI Official Symbol:LRWD1  
NCBI Official Synonym Symbols:ORCA; CENP-33  
NCBI Protein Information:leucine-rich repeat and WD repeat-containing protein 1; centromere protein 33; ORC-associated protein; origin recognition complex associated; origin recognition complex-associated protein
UniProt Protein Name:Leucine-rich repeat and WD repeat-containing protein 1
UniProt Synonym Protein Names:Centromere protein 33; CENP-33; Origin recognition complex-associated protein; ORC-associated protein; ORCA
Protein Family:Leucine-rich repeat and WD repeat-containing protein
UniProt Gene Name:LRWD1  
UniProt Entry Name:LRWD1_HUMAN
Anti-Lrwd1 Antibody (CAB15576)Western blot analysis of extracts of various cell lines, using Lrwd1 antibody (CAB15576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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