Name: Anti-LRPAP1 Antibody (CAB3004)
Brand: Cell Biology Antibodies 8
SKU: CAB3004
Availability: OutOfStock

Antibody Name:	Anti-LRPAP1 Antibody
Antibody SKU:	CAB3004
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human LRPAP1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, THP-1, HepG2

Immunogen:	A synthetic peptide of human LRPAP1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4043
Uniprot:	P30533
Cellular Location:	Cell surface, Cytoplasm, Endoplasmic reticulum
Calculated MW:	41kDa
Observed MW:	39kDa

Synonyms:	LRPAP1, A2MRAP, A2RAP, HBP44, MRAP, MYP23, RAP, alpha-2-MRAP
Background:	This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.
UniProt Protein Details:	Protein type:Receptor, misc. Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: cell surface; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; vesicle Molecular Function:heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding Disease: Myopia 23, Autosomal Recessive
NCBI Summary:	This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:	P30533
NCBI GenInfo Identifier:	231539
NCBI Gene ID:	4043
NCBI Accession:	P30533.1
UniProt Related Accession:	P30533
Molecular Weight:
NCBI Full Name:	Alpha-2-macroglobulin receptor-associated protein
NCBI Synonym Full Names:	LDL receptor related protein associated protein 1
NCBI Official Symbol:	LRPAP1
NCBI Official Synonym Symbols:	RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
NCBI Protein Information:	alpha-2-macroglobulin receptor-associated protein
UniProt Protein Name:	Alpha-2-macroglobulin receptor-associated protein
UniProt Synonym Protein Names:	Low density lipoprotein receptor-related protein-associated protein 1; RAP
UniProt Gene Name:	LRPAP1
UniProt Entry Name:	AMRP_HUMAN

	Western blot analysis of extracts of various cell lines, using LRPAP1 antibody (CAB3004) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using LRPAP1 Antibody (CAB3004) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using LRPAP1 Antibody (CAB3004) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using LRPAP1 Antibody (CAB3004) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using LRPAP1 Polyclonal Antibody (CAB3004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.