Antibody Name:	Anti-LRP8 Antibody
Antibody SKU:	CAB10517
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human LRP8 (NP_004622.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human LRP8 (NP_004622.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PLWA EPCL GETR EPED PAPA LKEL FVLP GEPR SQLH QLPK NPLS ELPV VKSK RVAL SLED DGLP
Gene ID:	7804
Uniprot:	Q14114
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	77kDa/87kDa/99kDa/105kDa
Observed MW:	105-120kDa

Synonyms:

LRP8, APOER2, HSZ75190, LRP-8, MCI1

Background:

This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
NCBI Summary:	This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2011]
UniProt Code:	Q14114
NCBI GenInfo Identifier:	259016389
NCBI Gene ID:	7804
NCBI Accession:	Q14114.4
UniProt Secondary Accession:	Q14114,O14968, Q86V27, Q99876, Q9BR78, B1AMT6, B1AMT7 B1AMT8,
UniProt Related Accession:	Q14114
Molecular Weight:	106kDa
NCBI Full Name:	Low-density lipoprotein receptor-related protein 8
NCBI Synonym Full Names:	LDL receptor related protein 8
NCBI Official Symbol:	LRP8
NCBI Official Synonym Symbols:	MCI1; LRP-8; APOER2; HSZ75190
NCBI Protein Information:	low-density lipoprotein receptor-related protein 8
UniProt Protein Name:	Low-density lipoprotein receptor-related protein 8
UniProt Synonym Protein Names:	Apolipoprotein E receptor 2
Protein Family:	Low-density lipoprotein receptor-related protein
UniProt Gene Name:	LRP8

	Western blot analysis of extracts of various cell lines, using LRP8 antibody (CAB10517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of Human placenta using LRP8 antibody (CAB10517) at dilution of 1:100. Blue: DAPI for nuclear staining.