Metabolism Antibodies 3
Anti-LPIN1 Antibody (CAB8486)
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- SKU:
- CAB8486
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-LPIN1 Antibody |
| Antibody SKU: | CAB8486 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 741-890 of human LPIN1 (NP_663731.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 741-890 of human LPIN1 (NP_663731.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NERG TVLP QGPL LLSP SSLF SALH REVI EKKP EKFK VQCL TDIK NLFF PNTE PFYA AFGN RPAD VYSY KQVG VSLN RIFT VNPK GELV QEHA KTNI SSYV RLCE VVDH VFPL LKRS HSSD FPCS DTFS NFTF WREP LPPF ENQD IHSA SA |
| Gene ID: | 23175 |
| Uniprot: | Q14693 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum membrane, Nucleus membrane, cytosol |
| Calculated MW: | 50kDa/98kDa/99-107kDa |
| Observed MW: | 113kDa |
| Synonyms: | LPIN1, PAP1, lipin 1 |
| Background: | This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their full-length structures have not been determined. |
| UniProt Protein Function: | LPIN1: Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. Interacts (via LXXIL motif) with PPARA. Interacts with PPARGC1A. Interaction with PPARA and PPARGC1A leads to the formation of a complex that modulates gene transcription. Interacts with MEF2C. Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract. Inhibited by N-ethylmaleimide. Belongs to the lipin family. |
| UniProt Protein Details: | Protein type:Phosphatase, lipid; EC 3.1.3.4 Chromosomal Location of Human Ortholog: 2p25.1 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; transcription factor complex; mitochondrial outer membrane; nuclear membrane; cytoplasm; nuclear envelope; nucleus; cytosol Molecular Function:peroxisome proliferator activated receptor binding; phosphatidate phosphatase activity; histone deacetylase binding; transcription coactivator activity Biological Process: mitochondrial fission; organ regeneration; transcription, DNA-dependent; regulation of fat cell differentiation; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; mitotic nuclear envelope disassembly; triacylglycerol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; fatty acid catabolic process; cellular lipid metabolic process; positive regulation of histone deacetylation; cellular response to insulin stimulus; dephosphorylation; phospholipid metabolic process; actin cytoskeleton reorganization; phosphatidylcholine biosynthetic process; ruffle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; triacylglycerol mobilization Disease: Myoglobinuria, Acute Recurrent, Autosomal Recessive |
| NCBI Summary: | This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their full-length structures have not been determined. [provided by RefSeq, May 2012] |
| UniProt Code: | Q14693 |
| NCBI GenInfo Identifier: | 387528011 |
| NCBI Gene ID: | 23175 |
| NCBI Accession: | NP_001248356.1 |
| UniProt Secondary Accession: | Q14693,Q53T25, A8MU38, B4DET9, B4DGS4, B4DGZ6, B5MC18 B7Z858, D6W506, E7ESE7, F5GY24, |
| UniProt Related Accession: | Q14693 |
| Molecular Weight: | 98,664 Da |
| NCBI Full Name: | phosphatidate phosphatase LPIN1 isoform 2 |
| NCBI Synonym Full Names: | lipin 1 |
| NCBI Official Symbol: | LPIN1 |
| NCBI Official Synonym Symbols: | PAP1 |
| NCBI Protein Information: | phosphatidate phosphatase LPIN1; lipin-1 |
| UniProt Protein Name: | Phosphatidate phosphatase LPIN1 |
| UniProt Synonym Protein Names: | Lipin-1 |
| Protein Family: | Phosphatidate phosphatase |
| UniProt Gene Name: | LPIN1 |
| UniProt Entry Name: | LPIN1_HUMAN |
 | Western blot analysis of extracts of rat skeletal muscle, using LPIN1 antibody (CAB8486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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