Cell Biology Antibodies 7
Anti-LMO2 Antibody (CAB1903)
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- SKU:
- CAB1903
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LMO2 Antibody |
| Antibody SKU: | CAB1903 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human LMO2 (NP_001135788.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, 293T, Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human LMO2 (NP_001135788.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSA IERK SLDP SEEP VDEV LQIP PSLL TCGG CQQN IGDR YFLK AIDQ YWHE DCLS CDLC GCRL GEVG RRLY YKLG RKLC RRDY LRLF GQDG LCAS CDKR IRAY EMTM RVKD KVYH LECF KCAA CQKH FCVG DRYL LINS DIVC EQDI YEWT KING MI |
| Gene ID: | 4005 |
| Uniprot: | P25791 |
| Cellular Location: | Nucleus |
| Calculated MW: | 5kDa/18kDa/25kDa |
| Observed MW: | 18kDa |
| Synonyms: | LMO2, RBTN2, RBTNL1, RHOM2, TTG2 |
| Background: | LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | LMO2: Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state. A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Oncoprotein Chromosomal Location of Human Ortholog: 11p13 Cellular Component: transcription factor complex; nucleus Molecular Function:protein binding; zinc ion binding; bHLH transcription factor binding; chromatin binding; cofactor binding Biological Process: negative regulation of erythrocyte differentiation; embryonic hemopoiesis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter |
| NCBI Summary: | LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008] |
| UniProt Code: | P25791 |
| NCBI GenInfo Identifier: | 132533 |
| NCBI Gene ID: | 4005 |
| NCBI Accession: | P25791.1 |
| UniProt Related Accession: | P25791 |
| Molecular Weight: | 18.4kDa |
| NCBI Full Name: | Rhombotin-2 |
| NCBI Synonym Full Names: | LIM domain only 2 |
| NCBI Official Symbol: | LMO2 |
| NCBI Official Synonym Symbols: | TTG2; LMO-2; RBTN2; RHOM2; RBTNL1 |
| NCBI Protein Information: | rhombotin-2 |
| UniProt Protein Name: | Rhombotin-2 |
| UniProt Synonym Protein Names: | Cysteine-rich protein TTG-2; LIM domain only protein 2; LMO-2; T-cell translocation protein 2 |
| Protein Family: | Rhombotin |
| UniProt Gene Name: | LMO2 |
| UniProt Entry Name: | RBTN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LMO2 antibody (CAB1903) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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