Cell Biology Antibodies 10

Anti-LMNB2 Antibody (CAB6483)

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SKU:
CAB6483
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LMNB2 Antibody
Antibody SKU:CAB6483
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, MCF7, SW620, Mouse liver, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AMAG ERDK FRKM LDAK EQEM TEMR DVMQ QQLA EYQE LLDV KLAL DMEI NAYR KLLE GEEE RLKL SPSP SSRV TVSR ATSS SSGS LSAT GRLG RSKR KRLE VEEP LGSG PSVL GTGT GGSG GFHL AQQA SASG SVSI EEID LEGK FVQL KNNS DKDQ SLGN WRIK RQVL EGEE IAYK FTPK YILR AGQM VTVW AAGA GVAH SPPS TLVW KGQS SWGT GESF RTVL VNAD GEEV AMRT VKKS SVMR ENEN GEEE EEEA EFGE EDLF HQQG DPRT TSRG CYVM
Gene ID:84823
Uniprot:Q03252
Cellular Location:Lipid-anchor, Nucleoplasmic side, Nucleus inner membrane
Calculated MW:69kDa
Observed MW:70kDa
Synonyms:LMNB2, EPM9, LAMB2, LMN2, lamin-B2
Background:This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.
UniProt Protein Function:Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: lamin filament; nuclear inner membrane

Molecular Function:structural molecule activity

Disease: Lipodystrophy, Partial, Acquired, Susceptibility To

UniProt Code:Q03252
NCBI GenInfo Identifier:23503078
NCBI Gene ID:
NCBI Accession:Q03252.3
Molecular Weight:
NCBI Full Name:Lamin-B2
UniProt Protein Name:Lamin-B2
Protein Family:Lamin
UniProt Gene Name:LMNB2  
UniProt Entry Name:LMNB2_HUMAN
Anti-LMNB2 Antibody (CAB6483)Western blot analysis of extracts of various cell lines, using LMNB2 antibody (CAB6483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-LMNB2 Antibody (CAB6483)Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens).
Anti-LMNB2 Antibody (CAB6483)Immunohistochemistry of paraffin-embedded human adenomyosis using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens).
Anti-LMNB2 Antibody (CAB6483)Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens).
Anti-LMNB2 Antibody (CAB6483)Immunofluorescence analysis of U2OS cells using LMNB2 antibody (CAB6483). Blue: DAPI for nuclear staining.
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