Cell Biology Antibodies 7
Anti-LMNB1 Antibody (CAB16685)
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- SKU:
- CAB16685
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LMNB1 Antibody |
| Antibody SKU: | CAB16685 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human LMNB1 |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, MCF7, mouse brain, mouse spleen |
| Immunogen: | Recombinant protein of human LMNB1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4001 |
| Uniprot: | P20700 |
| Cellular Location: | Lipid-anchor, Nucleoplasmic side, Nucleus inner membrane |
| Calculated MW: | 66kDa |
| Observed MW: | 70kDa |
| Synonyms: | ADLD, LMN, LMN2, LMNB, Lamin B1, LMNB1, lamin-B1 |
| Background: | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 5q23.2 Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope Molecular Function:phospholipase binding; structural molecule activity Biological Process: apoptosis; cell structure disassembly during apoptosis Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant |
| NCBI Summary: | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P20700 |
| NCBI GenInfo Identifier: | 125953 |
| NCBI Gene ID: | 4001 |
| NCBI Accession: | P20700.2 |
| UniProt Related Accession: | P20700 |
| Molecular Weight: | ~ 68kDa |
| NCBI Full Name: | Lamin-B1 |
| NCBI Synonym Full Names: | lamin B1 |
| NCBI Official Symbol: | LMNB1 |
| NCBI Official Synonym Symbols: | LMN; ADLD; LMN2; LMNB |
| NCBI Protein Information: | lamin-B1 |
| UniProt Protein Name: | Lamin-B1 |
| Protein Family: | Light-harvesting protein |
| UniProt Gene Name: | LMNB1 |
| UniProt Entry Name: | LMNB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (CAB16685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Confocal immunofluorescence analysis of HeLa cells using LMNB1 antibody (CAB16685) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of MCF-7 cells using LMNB1 antibody (CAB16685) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
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