Cell Biology Antibodies 16
Anti-LLGL2 Antibody (CAB13323)
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- SKU:
- CAB13323
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LLGL2 Antibody |
| Antibody SKU: | CAB13323 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of mouse LLGL2 (NP_663413.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse lung, Mouse testis, Rat lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of mouse LLGL2 (NP_663413.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IASC VFTK YGQG FYLI SPSE FERF SLST KWLV EPRC LVDS TKAK KHNR PSNG NGTG LKMT SSGH VRNS KSQS DGDE KKPG PVME HALL NDAW VLKE IQST L |
| Gene ID: | 217325 |
| Uniprot: | |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 114kDa |
| Observed MW: | 114kDa |
| Synonyms: | LLGL2, HGL, Hugl-2, LGL2 |
| Background: | The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. |
 | Western blot analysis of extracts of various cell lines, using LLGL2 antibody (CAB13323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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