Cell Biology Antibodies 6

Anti-LIN7A Antibody (CAB15346)

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SKU:
CAB15346
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LIN7A Antibody
Antibody SKU:CAB15346
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human LIN7A (NP_004655.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, MCF7, Mouse kidney, Mouse eye, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human LIN7A (NP_004655.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLKP SVTS APTA DMAT LTVV QPLT LDRD VARA IELL EKLQ ESGE VPVH KLQS LKKV LQSE FCTA IREV YQYM HETI TVNG CPEF RARA TA
Gene ID:8825
Uniprot:O14910
Cellular Location:Basolateral cell membrane, Cell junction, Cell membrane, Peripheral membrane protein, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome, tight junction
Calculated MW:25kDa
Observed MW:23-30kDa
Synonyms:LIN7A, LIN-7A, LIN7, MALS-1, TIP-33, VELI1
Background:The protein encoded by this gene is involved in generating and maintaining the asymmetric distribution of channels and receptors at the cell membrane. The encoded protein also is required for the localization of some specific channels and can be part of a protein complex that couples synaptic vesicle exocytosis to cell adhesion in the brain.
UniProt Protein Function:LIN7A: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. Belongs to the lin-7 family.
UniProt Protein Details:

Protein type:Cell adhesion; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: basolateral plasma membrane; intercellular junction; plasma membrane

Molecular Function:protein binding

Biological Process: maintenance of epithelial cell polarity; neurotransmitter secretion; protein complex assembly

NCBI Summary:The protein encoded by this gene is involved in generating and maintaining the asymmetric distribution of channels and receptors at the cell membrane. The encoded protein also is required for the localization of some specific channels and can be part of a protein complex that couples synaptic vesicle exocytosis to cell adhesion in the brain. [provided by RefSeq, May 2016]
UniProt Code:O14910
NCBI GenInfo Identifier:59798442
NCBI Gene ID:8825
NCBI Accession:O14910.2
UniProt Secondary Accession:O14910,Q147W1, Q6LES3, Q7LDS4, A4FTY3,
UniProt Related Accession:O14910
Molecular Weight:25,997 Da
NCBI Full Name:Protein lin-7 homolog A
NCBI Synonym Full Names:lin-7 homolog A, crumbs cell polarity complex component
NCBI Official Symbol:LIN7A  
NCBI Official Synonym Symbols:LIN7; VELI1; LIN-7A; MALS-1; TIP-33  
NCBI Protein Information:protein lin-7 homolog A
UniProt Protein Name:Protein lin-7 homolog A
UniProt Synonym Protein Names:Mammalian lin-seven protein 1; MALS-1; Tax interaction protein 33; TIP-33; Vertebrate lin-7 homolog 1; Veli-1
Protein Family:Protein
UniProt Gene Name:LIN7A  
UniProt Entry Name:LIN7A_HUMAN
Anti-LIN7A Antibody (CAB15346)Western blot analysis of extracts of various cell lines, using LIN7A antibody (CAB15346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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