Antibody Name:	Anti-LIMS1 Antibody
Antibody SKU:	CAB6384
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MANA LASA TCER CKGG FAPA EKIV NSNG ELYH EQCF VCAQ CFQQ FPEG LFYE FEGR KYCE HDFQ MLFA PCCH QCGE FIIG RVIK AMNN SWHP ECFR CDLC QEVL ADIG FVKN AGRH LCRP CHNR EKAR GLGK YICQ KCHA IIDE QPLI FKND PYHP DHFN CANC GKEL TADA RELK GELY CLPC HDKM GVPI CGAC RRPI EGRV VNAM GKQW HVEH FVCA KCEK PFLG HRHY ERKG LAYC ETHY NQLF GDVC FHCN RVIE GDVV SALN KAWC VNCF ACST CNTK LTLK NKFV EFDM KPVC KKCY EKFP LELK KRLK KLAE TLGR K
Gene ID:	3987
Uniprot:	P48059
Cellular Location:	Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, focal adhesion
Calculated MW:	37kDa/38kDa/41kDa/44kDa
Observed MW:	40kDa

Synonyms:

LIMS1, PINCH, PINCH-1, PINCH1

Background:

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	LIMS1: Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 2q12.3 Cellular Component: protein complex; focal adhesion; perinuclear region of cytoplasm; plasma membrane; intercellular junction; cytosol Molecular Function:protein binding; zinc ion binding; protein kinase binding Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; establishment of protein localization; chordate embryonic development; positive regulation of focal adhesion formation; protein heterooligomerization; cell-matrix adhesion; establishment and/or maintenance of cell polarity; cell aging; epithelial to mesenchymal transition; negative regulation of transcription, DNA-dependent; regulation of epithelial cell proliferation; positive regulation of GTPase activity; negative regulation of apoptosis
NCBI Summary:	The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	P48059
NCBI GenInfo Identifier:	18266876
NCBI Gene ID:	3987
NCBI Accession:	P48059.4
UniProt Secondary Accession:	P48059,Q53TE0, Q9BS44, B2RAJ4, B7Z483, B7Z7R3, B7Z907
UniProt Related Accession:	P48059
Molecular Weight:	325
NCBI Full Name:	LIM and senescent cell antigen-like-containing domain protein 1
NCBI Synonym Full Names:	LIM and senescent cell antigen-like domains 1
NCBI Official Symbol:	LIMS1
NCBI Official Synonym Symbols:	PINCH; PINCH1; PINCH-1
NCBI Protein Information:	LIM and senescent cell antigen-like-containing domain protein 1; renal carcinoma antigen NY-REN-48; particularly interesting new Cys-His protein 1
UniProt Protein Name:	LIM and senescent cell antigen-like-containing domain protein 1
UniProt Synonym Protein Names:	Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48
Protein Family:	LIM and senescent cell antigen-like-containing domain protein
UniProt Gene Name:	LIMS1
UniProt Entry Name:	LIMS1_HUMAN

	Western blot analysis of extracts of HepG2 cells, using LIMS1 antibody (CAB6384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of MCF7 cells using LIMS1 antibody (CAB6384). Blue: DAPI for nuclear staining.