Cell Biology Antibodies 9

Anti-LIMK2 Antibody (CAB5409)

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SKU:
CAB5409
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LIMK2 Antibody
Antibody SKU:CAB5409
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2, HeLa, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSY LSVP AYFT SRDL FRCS ECQD SLTN WYYE KDGK LYCP KDYW GKFG EFCH GCSL LMTG PFMV AGEF KYHP ECFA CMSC KVII EDGD AYAL VQHA TLYC GKCH NEVV LAPM FERL STES VQEQ LPYS VTLI SMPA TTEG RRGF SVSV ESAC SNYA TTVQ VKEV NRMH ISPN NRNA IHPG DRIL EING TPVR TLRV EEVE DAIS QTSQ TLQL LIEH DPVS QRLD QLRL EARL APHM QNAG
Gene ID:3985
Uniprot:P53671
Cellular Location:Cytoplasm, Nucleus, Nucleus
Calculated MW:69kDa/72kDa/77kDa
Observed MW:80kDa
Synonyms:LIMK2
Background:There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified.
UniProt Protein Details:

Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cis-Golgi network; cytoplasm; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding

Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation

NCBI Summary:There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P53671
NCBI GenInfo Identifier:1708824
NCBI Gene ID:3985
NCBI Accession:P53671.1
UniProt Secondary Accession:P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5
UniProt Related Accession:P53671
Molecular Weight:638
NCBI Full Name:LIM domain kinase 2
NCBI Synonym Full Names:LIM domain kinase 2
NCBI Official Symbol:LIMK2  
NCBI Protein Information:LIM domain kinase 2
UniProt Protein Name:LIM domain kinase 2
Protein Family:LIM domain kinase
UniProt Gene Name:LIMK2  
UniProt Entry Name:LIMK2_HUMAN
Anti-LIMK2 Antibody (CAB5409)Western blot analysis of extracts of various cell lines, using LIMK2 antibody (CAB5409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-LIMK2 Antibody (CAB5409)Immunofluorescence analysis of L929 cells using LIMK2 Rabbit pAb (CAB5409) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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