Immunology Antibodies 1
Anti-LIME1 Antibody (CAB13719)
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- SKU:
- CAB13719
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-LIME1 Antibody |
| Antibody SKU: | CAB13719 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human LIME1 (NP_060276.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | K-562, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human LIME1 (NP_060276.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGLP VSWA PPAL WVLG CCAL LLSL WALC TACR RPED AVAP RKRA RRQR ARLQ GSAT AAEA SLLR RTHL CSLS KSDT RLHE LHRG PRSS RALR PASM DLLR PHWL EVSR DITG PQAA PSAF PHQE LPRA LPAA AATA GCAG LEAT YSNV GLAA LPGV SLAA SPVV AEYA RVQK RKGT HRSP QEPQ QGKT EVTP AAQV DVLY SRVC KPKR RDPG PTTD PLDP KGQG AILA LAGD LAYQ TLPL RALD VDSG PLEN VYES IREL GDPA GRSS TCGA GTPP ASSC PSLG RGWR PLPA SLP |
| Gene ID: | 54923 |
| Uniprot: | Q9H400 |
| Cellular Location: | Cell membrane, Single-pass type III membrane protein |
| Calculated MW: | 20kDa/31kDa |
| Observed MW: | 31kDa-35kDa |
| Synonyms: | LIME1, LIME, dJ583P15.4 |
| Background: | This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | LIME1: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Adaptor/scaffold Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: extracellular space |
| NCBI Summary: | This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
| UniProt Code: | Q9H400 |
| NCBI GenInfo Identifier: | 74752630 |
| NCBI Gene ID: | 54923 |
| NCBI Accession: | Q9H400.1 |
| UniProt Secondary Accession: | Q9H400,Q5JWJ2, Q6XYB3, Q9NX69, E1P5K5, E1P5K6, |
| UniProt Related Accession: | Q9H400 |
| Molecular Weight: | 20,841 Da |
| NCBI Full Name: | Lck-interacting transmembrane adapter 1 |
| NCBI Synonym Full Names: | Lck interacting transmembrane adaptor 1 |
| NCBI Official Symbol: | LIME1 |
| NCBI Official Synonym Symbols: | LIME; dJ583P15.4 |
| NCBI Protein Information: | lck-interacting transmembrane adapter 1 |
| UniProt Protein Name: | Lck-interacting transmembrane adapter 1 |
| UniProt Synonym Protein Names: | Lck-interacting molecule |
| Protein Family: | Lck-interacting transmembrane adapter |
| UniProt Gene Name: | LIME1 |
| UniProt Entry Name: | LIME1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LIME1 antibody (CAB13719) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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