Metabolism Antibodies 1
Anti-LIMA1 Antibody (CAB11682)
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- SKU:
- CAB11682
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-LIMA1 Antibody |
| Antibody SKU: | CAB11682 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-759 of human LIMA1 (NP_057441.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-759 of human LIMA1 (NP_057441.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DEIS KPEV PEDV DLDL KKLR RSSS LKER SRPF TVAA SFQS TSVK SPKT VSPP IRKG WSMS EQSE ESVG GRVA ERKQ VENA KASK KNGN VGKT TWQN KESK GETG KRSK EGHS LEME NENL VENG ADSD EDDN SFLK QQSP QEPK SLNW SSFV DNTF AEEF TTQN QKSQ DVEL WEGE VVKE LSVE EQIK RNRY YDED EDEE |
| Gene ID: | 51474 |
| Uniprot: | Q9UHB6 |
| Cellular Location: | Cell junction, Cytoplasm, cytoskeleton, focal adhesion |
| Calculated MW: | 51kDa/67kDa/85kDa |
| Observed MW: | 100kDa |
| Synonyms: | LIMA1, EPLIN, SREBP3 |
| Background: | This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. |
| UniProt Protein Function: | eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q13 Cellular Component: actin cytoskeleton; cleavage furrow; cytoplasm; focal adhesion; plasma membrane; stress fiber Molecular Function:actin filament binding; actin monomer binding; protein binding Biological Process: actin filament bundle formation; negative regulation of actin filament depolymerization; ruffle organization and biogenesis |
| NCBI Summary: | This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9UHB6 |
| NCBI GenInfo Identifier: | 20138067 |
| NCBI Gene ID: | 51474 |
| NCBI Accession: | Q9UHB6.1 |
| UniProt Secondary Accession: | Q9UHB6,Q2TAN7, Q59FE8, Q9BVF2, Q9H8J1, Q9HBN5, Q9NX96 Q9NXC3, Q9NXU6, Q9P0H8, Q9UHB5, B2RB09, |
| UniProt Related Accession: | Q9UHB6 |
| Molecular Weight: | 67,117 Da |
| NCBI Full Name: | LIM domain and actin-binding protein 1 |
| NCBI Synonym Full Names: | LIM domain and actin binding 1 |
| NCBI Official Symbol: | LIMA1 |
| NCBI Official Synonym Symbols: | EPLIN; SREBP3 |
| NCBI Protein Information: | LIM domain and actin-binding protein 1 |
| UniProt Protein Name: | LIM domain and actin-binding protein 1 |
| UniProt Synonym Protein Names: | Epithelial protein lost in neoplasm |
| Protein Family: | LIM domain and actin-binding protein |
| UniProt Gene Name: | LIMA1 |
| UniProt Entry Name: | LIMA1_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using LIMA1 antibody (CAB11682) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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