Cell Biology Antibodies 11

Anti-LIM2 Antibody (CAB7909)

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SKU:
CAB7909
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LIM2 Antibody
Antibody SKU:CAB7909
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human LIM2 (NP_085915.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, HepG2, Jurkat, Mouse liver, Mouse eye, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human LIM2 (NP_085915.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TDHW MQYR LSGS FAHQ GLWR YCLG NKCY LQTD SIGE PPGQ GPGR AWGK SRAD LGAQ GHLY SRWR TLRL KEGK GATQ AYWN ATRA FM
Gene ID:3982
Uniprot:P55344
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:19kDa/24kDa
Observed MW:20kDa
Synonyms:LIM2, CTRCT19, MP17, MP19
Background:This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MP20: Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis. Belongs to the PMP-22/EMP/MP20 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.4

Disease: Cataract 19

NCBI Summary:This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
UniProt Code:P55344
NCBI GenInfo Identifier:17433717
NCBI Gene ID:3982
NCBI Accession:P55344.2
UniProt Secondary Accession:P55344,Q6B083, Q9BXD0, Q9HAR5,
UniProt Related Accession:P55344
Molecular Weight:24,188 Da
NCBI Full Name:Lens fiber membrane intrinsic protein
NCBI Synonym Full Names:lens intrinsic membrane protein 2
NCBI Official Symbol:LIM2  
NCBI Official Synonym Symbols:MP17; MP19; CTRCT19  
NCBI Protein Information:lens fiber membrane intrinsic protein
UniProt Protein Name:Lens fiber membrane intrinsic protein
UniProt Synonym Protein Names:MP18; MP19; MP20
Protein Family:Lens fiber membrane intrinsic protein
UniProt Gene Name:LIM2  
UniProt Entry Name:LMIP_HUMAN
Anti-LIM2 Antibody (CAB7909)Western blot analysis of extracts of various cell lines, using LIM2 antibody (CAB7909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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