Immunology Antibodies 2

Anti-LILRB3 Antibody (CAB16103)

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SKU:
CAB16103
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-LILRB3 Antibody
Antibody SKU:CAB16103
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human LILRB3 (NP_001307889.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human LILRB3 (NP_001307889.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RSSN PHLL SFPS EPLE LMVS GHSG GSSL PPTG PPST PGGP EDQP LNPP GSGP QNGL GRYL EVLI GVSV AFVL LLFL LLFL LLLR QRHS KHRT SDQR KTDF Q
Gene ID:11025
Uniprot:O75022
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:69kDa/71kDa
Observed MW:69kDa
Synonyms:LILRB3, CD85A, HL9, ILT-5, ILT5, LILRA6, LIR-3, LIR3, PIR-B, PIRB
Background:This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Function: May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM)
UniProt Protein Details:

By similarity.

Subunit structure: Interacts with LYN, PTPN6/SHP-1 and PTPN11/SHP-2

By similarity. Ref.5

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Detected in monocytes and B-cells. Ref.2

Domain: Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, including PTPN6/SHP-1, resulting in the dephosphorylation of the downstream protein kinases SYK and BTK

By similarity.

Post-translational modification: Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2

By similarity.

Miscellaneous: Belongs to the leukocyte receptor cluster (LRC) present on 19q13.4.

Sequence similarities: Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

NCBI Summary:This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O75022
NCBI GenInfo Identifier:125661043
NCBI Gene ID:11025
NCBI Accession:NP_001074919.1
UniProt Secondary Accession:O75022,O15471, Q86U49, C9J1P3, C9JIP1,
UniProt Related Accession:O75022
Molecular Weight:69,386 Da
NCBI Full Name:leukocyte immunoglobulin-like receptor subfamily B member 3 isoform 1
NCBI Synonym Full Names:leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3
NCBI Official Symbol:LILRB3  
NCBI Official Synonym Symbols:HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; LILRA6  
NCBI Protein Information:leukocyte immunoglobulin-like receptor subfamily B member 3; immunoglobulin-like transcript 5; monocyte inhibitory receptor HL9; CD85 antigen-like family member A; leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6
UniProt Protein Name:Leukocyte immunoglobulin-like receptor subfamily B member 3
UniProt Synonym Protein Names:CD85 antigen-like family member A; Immunoglobulin-like transcript 5; ILT-5; Monocyte inhibitory receptor HL9
UniProt Gene Name:LILRB3  
UniProt Entry Name:LIRB3_HUMAN
Anti-LILRB3 Antibody (CAB16103)Western blot analysis of extracts of Jurkat cells, using LILRB3 antibody (CAB16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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