Antibody Name:	Anti-LIG4 Antibody
Antibody SKU:	CAB1743
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human
Positive Samples:	K-562, Jurkat, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SKHL YIGG DDEP QEKK RKAA PKMK KVIG IIEH LKAP NLTN VNKI SNIF EDVE FCVM SGTD SQPK PDLE NRIA EFGG YIVQ NPGP DTYC VIAG SENI RVKN IILS NKHD VVKP AWLL ECFK TKSF VPWQ PRFM IHMC PSTK EHFA REYD CYGD SYFI DTDL NQLK EVFS GIKN SNEQ TPEE MASL IADL EYRY SWDC SPLS MFRR HTVY LDSY AVIN DLST KNEG TRLA IKAL ELRF HGAK VVSC LAEG VSHV IIGE DHSR VADF KAFR RTFK RKFK ILKE SWVT DSID KCEL QEEN QYLI
Gene ID:	3981
Uniprot:	P49917
Cellular Location:	Nucleus
Calculated MW:	103kDa
Observed MW:	108kDa

Synonyms:

LIG4, LIG4S

Background:

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

UniProt Protein Function:	LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.
UniProt Protein Details:	Protein type:EC 6.5.1.1; Ligase Chromosomal Location of Human Ortholog: 13q33-q34 Cellular Component: condensed chromosome; cytoplasm; DNA ligase IV complex; DNA-dependent protein kinase complex; focal adhesion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane Molecular Function:ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; ligase activity; metal ion binding; protein binding; protein C-terminus binding Biological Process: cell cycle; cell division; cell proliferation; central nervous system development; chromosome organization and biogenesis; DNA ligation; DNA ligation during DNA recombination; DNA ligation during DNA repair; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; lagging strand elongation; negative regulation of neuron apoptosis; neuron apoptosis; nucleotide-excision repair, DNA gap filling; positive regulation of fibroblast proliferation; positive regulation of neurogenesis; pro-B cell differentiation; response to gamma radiation; response to X-ray; single strand break repair; somatic stem cell maintenance; T cell differentiation in the thymus; T cell receptor V(D)J recombination; V(D)J recombination; viral reproduction Disease: Lig4 Syndrome; Myeloma, Multiple
NCBI Summary:	The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	P49917
NCBI GenInfo Identifier:	88911290
NCBI Gene ID:	3981
NCBI Accession:	P49917.2
UniProt Secondary Accession:	P49917,Q8IY66, Q8TEU5,
UniProt Related Accession:	P49917
Molecular Weight:	103,971 Da
NCBI Full Name:	DNA ligase 4
NCBI Synonym Full Names:	ligase IV, DNA, ATP-dependent
NCBI Official Symbol:	LIG4
NCBI Official Synonym Symbols:	LIG4S
NCBI Protein Information:	DNA ligase 4
UniProt Protein Name:	DNA ligase 4
UniProt Synonym Protein Names:	DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Protein Family:	DNA ligase
UniProt Gene Name:	LIG4
UniProt Entry Name:	DNLI4_HUMAN

	Western blot analysis of extracts of various cell lines, using LIG4 antibody (CAB1743) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U-2OS cells using LIG4 antibody (CAB1743) at dilution of 1:100. Blue: DAPI for nuclear staining.