Cell Biology Antibodies 3

Anti-LIF Antibody (CAB1288)

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SKU:
CAB1288
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LIF Antibody
Antibody SKU:CAB1288
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-202 of human LIF (NP_002300.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human
Positive Samples:SH-SY5Y
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-202 of human LIF (NP_002300.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPLP ITPV NATC AIRH PCHN NLMN QIRS QLAQ LNGS ANAL FILY YTAQ GEPF PNNL DKLC GPNV TDFP PFHA NGTE KAKL VELY RIVV YLGT SLGN ITRD QKIL NPSA LSLH SKLN ATAD ILRG LLSN VLCR LCSK YHVG HVDV TYGP DTSG KDVF QKKK LGCQ LLGK YKQI IAVL AQAF
Gene ID:3976
Uniprot:P15018
Cellular Location:Secreted
Calculated MW:9kDa/22kDa
Observed MW:21kDa
Synonyms:LIF, CDF, DIA, HILDA, MLPLI
Background:The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
NCBI Summary:The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:P15018
NCBI GenInfo Identifier:126279
NCBI Gene ID:3976
NCBI Accession:P15018.1
UniProt Secondary Accession:P15018,Q52LZ2, B2RCW7, B5MC23,
UniProt Related Accession:P15018
Molecular Weight:9,563 Da
NCBI Full Name:Leukemia inhibitory factor
NCBI Synonym Full Names:LIF, interleukin 6 family cytokine
NCBI Official Symbol:LIF  
NCBI Official Synonym Symbols:CDF; DIA; HILDA; MLPLI  
NCBI Protein Information:leukemia inhibitory factor
UniProt Protein Name:Leukemia inhibitory factor
UniProt Synonym Protein Names:Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin
Protein Family:Leukemia inhibitory factor
UniProt Gene Name:LIF  
Anti-LIF Antibody (CAB1288)Western blot analysis of extracts of SH-SY5Y cells, using LIF antibody (CAB1288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-LIF Antibody (CAB1288)Immunohistochemistry of paraffin-embedded human prostate using LIF antibody (CAB1288) at dilution of 1:100 (40x lens).
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