Antibody Name:	Anti-LGR6 Antibody
Antibody SKU:	CAB7383
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-510 of human LGR6 (NP_067649.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-510 of human LGR6 (NP_067649.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALDL SWNA IRSI HPEA FSTL HSLV KLDL TDNQ LTTL PLAG LGGL MHLK LKGN LALS QAFS KDSF PKLR ILEV PYAY QCCP YGMC ASFF KASG QWEA EDLH LDDE ESSK RPLG LLAR QAEN HYDQ DLDE LQLE MEDS KPHP SVQC SPTP GPFK PCEY LFES W
Gene ID:	59352
Uniprot:	Q9HBX8
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	89kDa/99kDa/104kDa
Observed MW:	104kDa

Synonyms:

LGR6, GPCR, VTS20631

Background:

This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	LGR6: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of adult stem cells. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. A marker of a multipotent population of skin stem cells. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Tumor suppressor; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane; vesicle Molecular Function:G-protein coupled receptor activity; protein binding; transmembrane receptor activity Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cell migration; positive regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway
NCBI Summary:	This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9HBX8
NCBI GenInfo Identifier:	158519993
NCBI Gene ID:	59352
NCBI Accession:	Q9HBX8.3
UniProt Secondary Accession:	Q9HBX8,Q5T509, Q5T512, Q6UY15, Q86VU0, Q96K69, Q9BYD7
UniProt Related Accession:	Q9HBX8
Molecular Weight:	99,295 Da
NCBI Full Name:	Leucine-rich repeat-containing G-protein coupled receptor 6
NCBI Synonym Full Names:	leucine-rich repeat containing G protein-coupled receptor 6
NCBI Official Symbol:	LGR6
NCBI Official Synonym Symbols:	GPCR; VTS20631
NCBI Protein Information:	leucine-rich repeat-containing G-protein coupled receptor 6
UniProt Protein Name:	Leucine-rich repeat-containing G-protein coupled receptor 6
Protein Family:	Leucine-rich repeat-containing G-protein coupled receptor
UniProt Gene Name:	LGR6
UniProt Entry Name:	LGR6_HUMAN

	Western blot analysis of extracts of various cell lines, using LGR6 antibody (CAB7383) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using LGR6 Antibody (CAB7383) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using LGR6 Antibody (CAB7383) at dilution of 1:100 (40x lens).