Antibody Name:	Anti-LGMN Antibody
Antibody SKU:	CAB10570
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VPID DPED GGKH WVVI VAGS NGWY NYRH QADA CHAY QIIH RNGI PDEQ IVVM MYDD IAYS EDNP TPGI VINR PNGT DVYQ GVPK DYTG EDVT PQNF LAVL RGDA EAVK GIGS GKVL KSGP QDHV FIYF TDHG STGI LVFP NEDL HVKD LNET IHYM YKHK MYRK MVFY IEAC ESGS MMNH LPDN INVY ATTA ANPR ESSY ACYY DEKR STYL GDWY SVNW MEDS DVED LTKE TLHK QYHL VKSH TNTS HVMQ YGNK TIST MKVM QFQG MKRK ASSP VPLP PVTH LDLT PSPD VPLT IMKR KLMN TN
Gene ID:	5641
Uniprot:	Q99538
Cellular Location:	Lysosome
Calculated MW:	42kDa/49kDa
Observed MW:	54kDa

Synonyms:

LGMN, AEP, LGMN1, PRSC1, legumain

Background:

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

UniProt Protein Function:	LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.
UniProt Protein Details:	Protein type:EC 3.4.22.34; Protease Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: lysosomal lumen; lysosome Molecular Function:cysteine-type endopeptidase activity; peptidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process
NCBI Summary:	This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99538
NCBI GenInfo Identifier:	2842759
NCBI Gene ID:	5641
NCBI Accession:	Q99538.1
UniProt Secondary Accession:	Q99538,O00123, Q86TV2, Q86TV3, Q9BTY1,
UniProt Related Accession:	Q99538
Molecular Weight:	Observed MW: 54kDaCalculated MW: 42kDa/49kDa
NCBI Full Name:	Legumain
NCBI Synonym Full Names:	legumain
NCBI Official Symbol:	LGMN
NCBI Official Synonym Symbols:	AEP; LGMN1; PRSC1
NCBI Protein Information:	legumain
UniProt Protein Name:	Legumain
UniProt Synonym Protein Names:	Asparaginyl endopeptidase; Protease, cysteine 1
UniProt Gene Name:	LGMN

	Western blot analysis of extracts of various cell lines, using LGMN antibody (CAB10570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using LGMN Polyclonal Antibody (CAB10570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody (CAB10570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (CAB10570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.