KO Validated Antibodies 1

Anti-LGALS3 Antibody (CAB13506)[KO Validated]

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SKU:
CAB13506
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-LGALS3 Antibody [KO Validated]
Antibody SKU:CAB13506
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, SGC-7901, HeLa, Mouse brain, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADN FSLH DALS GSGN PNPQ GWPG AWGN QPAG AGGY PGAS YPGA YPGQ APPG AYPG QAPP GAYP GAPG AYPG APAP GVYP GPPS GPGA YPSS GQPS ATGA YPAT GPYG APAG PLIV PYNL PLPG GVVP RMLI TILG TVKP NANR IALD FQRG NDVA FHFN PRFN ENNR RVIV CNTK LDNN WGRE ERQS VFPF ESGK PFKI QVLV EPDH FKVA VNDA HLLQ YNHR VKKL NEIS KLGI SGDI DLTS ASYT MI
Gene ID:3958
Uniprot:P17931
Cellular Location:Cytoplasm, Nucleus, Secreted
Calculated MW:26kDa
Observed MW:31kDa
Synonyms:LGALS3, CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2
Background:This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Extracellular matrix; Cell surface

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: spliceosome; extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function:protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; epithelial cell differentiation; positive chemotaxis; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

NCBI Summary:This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
UniProt Code:P17931
NCBI GenInfo Identifier:215274262
NCBI Gene ID:3958
NCBI Accession:P17931.5
UniProt Secondary Accession:P17931,Q16005, Q6IBA7, Q96J47, B2RC38,
UniProt Related Accession:P17931
Molecular Weight:
NCBI Full Name:Galectin-3
NCBI Synonym Full Names:lectin, galactoside-binding, soluble, 3
NCBI Official Symbol:LGALS3  
NCBI Official Synonym Symbols:L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2  
NCBI Protein Information:galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
UniProt Protein Name:Galectin-3
UniProt Synonym Protein Names:35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
Protein Family:Galectin
UniProt Gene Name:LGALS3  
UniProt Entry Name:LEG3_HUMAN
Anti-LGALS3 Antibody [KO Validated] (CAB13506)Western blot analysis of extracts of various cell lines, using LGALS3 antibody (CAB13506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-LGALS3 Antibody [KO Validated] (CAB13506)Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (CAB13506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-LGALS3 Antibody [KO Validated] (CAB13506)Immunofluorescence analysis of C6 cells using LGALS3 antibody (CAB13506) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-LGALS3 Antibody [KO Validated] (CAB13506)Immunofluorescence analysis of L-929 cells using LGALS3 antibody (CAB13506) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-LGALS3 Antibody [KO Validated] (CAB13506)Immunofluorescence analysis of U-2 OS cells using LGALS3 antibody (CAB13506) at dilution of 1:100. Blue: DAPI for nuclear staining.
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