Antibody Name:	Anti-LFNG Antibody
Antibody SKU:	CAB7441
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, SW620, PC-12, HeLa, Mouse spleen, Mouse brain, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTPG RCCL AADI QVET FIFT DGED EALA RHTG NVVI TNCS AAHS RQAL SCKM AVEY DRFI ESGR KWFC HVDD DNYV NLRA LLRL LASY PHTR DVYV GKPS LDRP IQAM ERVS ENKV RPVH FWFA TGGA GFCI SRGL ALKM SPWA SGGH FMNT AERI RLPD DCTI GYIV EALL GVPL IRSG LFHS HLEN LQQV PTSE LHEQ VTLS YGMF ENKR NAVH VKGP FSVE ADPS RFRS IHCH LYPD TPWC PRTA IF
Gene ID:	3955
Uniprot:	Q8NES3
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	28kDa/35kDa/39kDa/41kDa
Observed MW:	42kDa

Synonyms:

LFNG, SCDO3, beta-1

Background:

This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1, 3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	LFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning. Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3). An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. Belongs to the glycosyltransferase 31 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Transferase; Membrane protein, integral; EC 2.4.1.222 Chromosomal Location of Human Ortholog: 7p22.2 Cellular Component: extracellular region; integral to Golgi membrane; vesicle Molecular Function:metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity Biological Process: compartment specification; somitogenesis; organ morphogenesis; Notch signaling pathway; regulation of Notch signaling pathway; positive regulation of protein binding; ovarian follicle development; metabolic process; female meiosis; regulation of somitogenesis; positive regulation of Notch signaling pathway Disease: Spondylocostal Dysostosis 3, Autosomal Recessive
NCBI Summary:	This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	Q8NES3
NCBI GenInfo Identifier:	27734417
NCBI Gene ID:	3955
NCBI Accession:	Q8NES3.2
UniProt Secondary Accession:	Q8NES3,O00589, Q96C39, Q9UJW5, B3KTY6, B5MCR5,
UniProt Related Accession:	Q8NES3
Molecular Weight:	379
NCBI Full Name:	Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
NCBI Synonym Full Names:	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol:	LFNG
NCBI Official Synonym Symbols:	SCDO3
NCBI Protein Information:	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
UniProt Protein Name:	Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
UniProt Synonym Protein Names:	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Protein Family:	Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
UniProt Gene Name:	LFNG
UniProt Entry Name:	LFNG_HUMAN

	Western blot analysis of extracts of various cell lines, using LFNG antibody (CAB7441) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using LFNG antibody (CAB7441) at dilution of 1:100 (40x lens).