Cell Biology Antibodies 11
Anti-LEPREL2 Antibody (CAB8062)
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- SKU:
- CAB8062
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | LEPREL2 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8062 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 487-736 of human LEPREL2 (NP_055077.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 22Rv1, NIH/3T3, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 487-736 of human LEPREL2 (NP_055077.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DAAG AGAR SGYR GRRS PHTP HERF EGLT VLKA AQLA RAGT VGSQ GAKL LLEV SERV RTLT QAYF SPER PLHL SFTH LVCR SAIE GEQE QRMD LSHP VHAD NCVL DPDT GECW REPP AYTY RDYS GLLY LNDD FQGG DLFF TEPN ALTV TARV RPRC GRLV AFSS GVEN PHGV WAVT RGRR CALA LWHT WAPE HREQ EWIE AKEL LQES QEEE EEEE EEMP SKDP SPEP PSRR HQRV QDKT GRAP RVRE EL |
| Gene ID: | 10536 |
| Uniprot: | Q8IVL6 |
| Cellular Location: | Endoplasmic reticulum |
| Calculated MW: | 62kDa/81kDa |
| Observed MW: | 82kDa |
| Synonyms: | P3H3, GRCB, HSU47926, LEPREL2 |
| Background: | The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor. |
| UniProt Protein Function: | LEPREL2: Has prolyl 3-hydroxylase activity catalyzing the post- translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. Belongs to the leprecan family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.7; Oxidoreductase Chromosomal Location of Human Ortholog: 12p13.31 Cellular Component: endoplasmic reticulum lumen Molecular Function:procollagen-proline 3-dioxygenase activity; L-ascorbic acid binding; iron ion binding Biological Process: negative regulation of cell proliferation; extracellular matrix organization and biogenesis; peptidyl-proline hydroxylation |
| NCBI Summary: | The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q8IVL6 |
| NCBI GenInfo Identifier: | 74714366 |
| NCBI Gene ID: | 10536 |
| NCBI Accession: | Q8IVL6.1 |
| UniProt Related Accession: | Q8IVL6 |
| Molecular Weight: | 82kDa |
| NCBI Full Name: | Prolyl 3-hydroxylase 3 |
| NCBI Synonym Full Names: | prolyl 3-hydroxylase 3 |
| NCBI Official Symbol: | P3H3 |
| NCBI Official Synonym Symbols: | GRCB; LEPREL2; HSU47926 |
| NCBI Protein Information: | prolyl 3-hydroxylase 3 |
| UniProt Protein Name: | Prolyl 3-hydroxylase 3 |
| UniProt Synonym Protein Names: | Leprecan-like protein 2; Protein B |
| UniProt Gene Name: | LEPREL2 |
| UniProt Entry Name: | P3H3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LEPREL2 antibody (CAB8062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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